June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Effects of chronic intraocular pressure elevation on cerebrospinal fluid dynamics in the optic nerve
Author Affiliations & Notes
  • Muneeb A Faiq
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Vishnu Adi
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Jingyuan Zhu
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China
  • Sophia Khoja
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Anoop Sainulabdeen
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Carlos Parra
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Giles Hamilton-Fletcher
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Choong H Lee
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Jiangyang Zhang
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Christopher Kai-Shun Leung
    Department of Ophthalmology, The Chinese University of Hong Kong, Hong Kong, China
  • Gadi Wollstein
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, New York, United States
  • Joel S Schuman
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, New York, United States
  • Kevin Chan
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Footnotes
    Commercial Relationships   Muneeb Faiq, None; Vishnu Adi, None; Jingyuan Zhu, None; Sophia Khoja, None; Anoop Sainulabdeen, None; Carlos Parra, None; Giles Hamilton-Fletcher, None; Choong Lee, None; Jiangyang Zhang, None; Christopher Leung, None; Gadi Wollstein, None; Joel Schuman, None; Kevin Chan, None
  • Footnotes
    Support  This work was supported in part by the National Institutes of Health R01-EY028125, P30-CA016087 and P41-EB017183 (Bethesda, Maryland); BrightFocus Foundation G2019103 (Clarksburg, Maryland); Feldstein Medical Foundation Research Grant (Clifton, New Jersey); Research to Prevent Blindness/Stavros Niarchos Foundation International Research Collaborators Award (New York, New York); and an unrestricted grant from Research to Prevent Blindness to NYU Langone Health Department of Ophthalmology (New York, New York).
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1861. doi:
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    • Get Citation

      Muneeb A Faiq, Vishnu Adi, Jingyuan Zhu, Sophia Khoja, Anoop Sainulabdeen, Carlos Parra, Giles Hamilton-Fletcher, Choong H Lee, Jiangyang Zhang, Christopher Kai-Shun Leung, Gadi Wollstein, Joel S Schuman, Kevin Chan; Effects of chronic intraocular pressure elevation on cerebrospinal fluid dynamics in the optic nerve. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1861.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Though chronic intraocular pressure (IOP) elevation and glymphatic disturbances have been implicated in various optic neuropathies, the relation between IOP and optic nerve (ON) cerebrospinal fluid (CSF) dynamics has not been sufficiently investigated. We employed contrast-enhanced MRI (CE-MRI) for in vivo investigation of CSF dynamics in the ON with and without chronic IOP elevation.

Methods : IOP was elevated unilaterally in adult C57BL/6J mice (n=15, Group 1) by intracameral injection of in situ cross-linking hydrogel. Chronic IOP elevation in the injected eye (Group 1a) was confirmed at 4 weeks post-injection, at 19.37±3.41 mmHg (mean±SEM) relative to the fellow, untreated eye (Group 1b) at 11.39±3.74 mmHg. Another 20 healthy naive mice received no intervention to either eye (Group 2). A polyethylene tubing was inserted intrathecally at the lumbar region (L4-L5) followed by CE-MRI using a 7-Tesla Bruker BioSpec scanner. Contrast dynamics was monitored using 3D T1-weighted FLASH sequence with an isotropic resolution of 78x78x78 μm3. Twelve 10-min continuous scans were acquired, beginning with 3 baseline acquisitions followed by 30 min of Gd-DTPA contrast infusion while the scanning continued till the 12 timepoint. Change in contrast intensity (CI) over time was quantified in 6 anatomical regions of interest (ROIs) viz. posterior ON subarachnoid space (ONSAS-P), anterior ON SAS (ONSAS-A), posterior ON parenchyma (ONP-P), anterior ON parenchyma (ONP-A), olfactory bulb (OB, positive control), and muscle tissue (MT, negative control).

Results : Between group ANOVAs were significant for each ROI in the ON (all p’s<.001), and not significant for control regions. Post-hocs revealed that for ONSAS-A, Group 1a had lower CI than Group 1b (p<.001) and Group 2 (p<.001), while Groups 1b and 2 also differed (p=.011). For ONP-A, Group 1a had lower CI than Group 1b (p=.003) and Group 2 (p<.001), with no difference between Groups 1b and 2. For ONSAS-P, Groups 1b and 2 CI differed significantly (p=.011), and for ONP-P, Group 1a had lower CI than Group 1b (p<.001) and Group 2 (p=.003), while Groups 1b and 2 CI also differed (p=.004).

Conclusions : Chronic IOP elevation is associated with significant decrease in the CSF flow into the ON SAS and ON parenchyma. CSF dynamics is also altered in the contralateral ON projected from the fellow eye with normal IOP.

This is a 2021 ARVO Annual Meeting abstract.

 

 

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