June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Clinical retinal oximetry with visible-light optical coherence tomography
Author Affiliations & Notes
  • Ian Rubinoff
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Roman V. Kuranov
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
    Opticent Health, Evanston, Illinois, United States
  • Yuanbo Wang
    Opticent Health, Evanston, Illinois, United States
  • Zeinab Ghassabi
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Gadi Wollstein
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Behnam Tayebi
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Hiroshi Ishikawa
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Joel S Schuman
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Hao Zhang
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
    Opticent Health, Evanston, Illinois, United States
  • Footnotes
    Commercial Relationships   Ian Rubinoff, None; Roman V. Kuranov, Opticent Health (E); Yuanbo Wang, Opticent Health (E); Zeinab Ghassabi, None; Gadi Wollstein, None; Behnam Tayebi, None; Hiroshi Ishikawa, None; Joel Schuman, Opticent Health (I); Hao Zhang, Opticent Health (I)
  • Footnotes
    Support  NIH Grants R01EY026078, R01EY029121, R44EY026466, R01EY013178, and unrestricted grant from Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 27. doi:
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      Ian Rubinoff, Roman V. Kuranov, Yuanbo Wang, Zeinab Ghassabi, Gadi Wollstein, Behnam Tayebi, Hiroshi Ishikawa, Joel S Schuman, Hao Zhang; Clinical retinal oximetry with visible-light optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2021;62(8):27.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Alterations in retinal oxygen metabolism are implicated in blindness causing diseases, such as diabetic retinopathy and glaucoma. Therefore, a non-invasive clinical tool to assess oxygen saturation (sO2) in retinal vessels is desirable. Recent development of visible-light optical coherence tomography (vis-OCT) enabled non-invasive sO2 measurements in retinal blood vessels at micrometer-scale resolution by three-dimensional (3D) spectroscopic analysis. Nevertheless, such measurements are susceptible to spectral contaminants from the complex retina anatomy and vis-OCT signal detection and processing, decreasing measurement reliability. To overcome limitations posed by spectral contaminants, we developed adaptive-spectroscopic OCT (AS-OCT), a processing technique that enables non-invasive, 3D, environment-independent sO2 measurements in the human retina.

Methods : We used vis-OCT to image the retinas of 18 healthy volunteers. Light exposure in the eye was < 250 μW and imaging acquisition time was 5 sec. We used adaptive spectroscopic OCT (AS-OCT) to identify and remove contaminants from retinal tissues, chromatic aberrations, and spectrally-dependent roll-off. Then, we automatically selected the optimal depths in the vessel for sO2 measurement. Finally, we measured the attenuation spectrum in the blood vessel and used a least-squares regression fit with known spectra to determine the sO2 value.

Results : We measured sO2 in 125 unique retinal vessels near the optic disc (vessel diameters ranging from 37 μm to 176 μm). Major arteries had sO2 = 97.9 +/- 2.9 % (mean +/- standard deviation) (n = 36), small arteries (diameter < 100 had sO2 = 93.2 +/- 5.0 % (n = 36), and veins had sO2 = 58.5 +/- 4.3 % (n = 53). Repeated measurement standard deviations were 2.21% and 2.32% for all arteries and veins, respectively. Fig. 1 shows an oximetry map of the optic disc in the retina of a healthy 23 year-old volunteer.

Conclusions : AS-OCT enables environment-independent retinal oximetry in the clinical setting. Repeatability in arteries and veins < 2.5 % indicates robust measurements that are promising for clinical use.

This is a 2021 ARVO Annual Meeting abstract.

 

Figure 1. (a) Oximetry map of optic disc in 23-year-old volunteer. The image range is 4.8 x 4.8 mm2.

Figure 1. (a) Oximetry map of optic disc in 23-year-old volunteer. The image range is 4.8 x 4.8 mm2.

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