June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Capillary Flow Characteristics Vary with Hematocrit on SS-OCTA
Author Affiliations & Notes
  • Sam Kushner-Lenhoff
    Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, California, United States
  • Yuandong Li
    Bioengineering and Ophthalmology, University of Washington, Seattle, Washington, United States
  • Qinqin Zhang
    Bioengineering and Ophthalmology, University of Washington, Seattle, Washington, United States
  • Ruikang K Wang
    Bioengineering and Ophthalmology, University of Washington, Seattle, Washington, United States
  • Xuejuan Jiang
    Ophthalmology, USC Roski Eye Institute, University of Southern California, Los Angeles, California, United States
  • Amir H Kashani
    Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Sam Kushner-Lenhoff, None; Yuandong Li, None; Qinqin Zhang, None; Ruikang Wang, Carl Zeiss Meditec (P), Carl Zeiss Meditec (C), Insight Photonic Solutions (C), Kowa Inc (P); Xuejuan Jiang, None; Amir Kashani, Carl Zeiss Meditec (F), Carl Zeiss Meditec (R)
  • Footnotes
    Support  Dr. Jiang: National Eye Institute Grant/Award Numbers: U10 EY023575 and R21EY028721. Dr. Kashani: K08EY027006, R01EY030564. Unrestricted departmental funding from Research to Prevent Blindness (New York, NY, USA); Research Grants from Carl Zeiss Meditec, Inc. (Dublin, CA, USA), Carl Zeiss Meditec, Inc. was not consulted in the design, implementation, or analysis of the study data.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2478. doi:
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    • Get Citation

      Sam Kushner-Lenhoff, Yuandong Li, Qinqin Zhang, Ruikang K Wang, Xuejuan Jiang, Amir H Kashani; Capillary Flow Characteristics Vary with Hematocrit on SS-OCTA. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2478.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To examine the associations of capillary density and flow with hematocrit, blood pressure and medical comorbidities using OCTA.

Methods : A cross-sectional study using 3 x 3 mm SS-OCTA images to measure vessel skeleton density (VSD) and flow (flux) in the superficial retinal layer (SRL). Flux is a relatively new measure that approximates the number of red blood cells moving through a vessel segment per unit time. Complete blood count, blood pressure, self-reported/medical-record based history including diabetes status, hypertension status, hypertensive medication use, cigarette smoking and retinopathy status (including edema) were obtained. A generalized linear mixed-effects model was used to evaluate the relationship with OCTA parameters after considering the correlation between eyes. Least-square means were estimated.

Results : A total of 154 eyes from 83 participants [56 women and 27 men, mean [SD] age, 66.2 (8.9) years, 22 with diabetes, 63 with hypertension] were included. Mean VSD was 0.147 ± 0.009 and mean flux was 0.156 ± 0.016. VSD showed a negative correlation with age (p=.001) and retinopathy (p=.016), but no significant correlation with hematocrit (p=.86) or signal strength (p=.51). Flux showed a positive correlation with hematocrit (p = .006) and signal strength (p<.001), as well as a negative correlation with age (p=.005) and diabetes status (p=.050). These associations remained even after accounting for hypertensive status, and smoking status. Mean flux was 0.010 lower in OCTA scans with a signal strength of 9 compared with those with a strength of 10, and 0.006 lower for the following comparisons: participants age 65 and above with younger participants, participants having hematocrit of less than 40% with those having higher hematocrit, and diabetics with non-diabetics. A one percent decrease in hematocrit was approximately equivalent to 1.8 years of aging in its effect on the calculated flux.

Conclusions : Retinal blood flow is independently affected by hematocrit and diabetes status even when accounting for known determinants of capillary density such as age and retinopathy status. This effect by hematocrit is not found in OCTA measurements of vessel skeletal density.

This is a 2021 ARVO Annual Meeting abstract.

 

Vessel flux from the right eyes of two female participants with OCTA image signal strength of 10.

Vessel flux from the right eyes of two female participants with OCTA image signal strength of 10.

 

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