June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
AO-OCT imaging of the retina with a pyramid wavefront sensor
Author Affiliations & Notes
  • Elisabeth Brunner
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Julia Shatokhina
    Johann Radon Institute for Computational and Applied Mathematics, Linz, Oberösterreich, Austria
  • Muhammad Faizan Shirazi
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Wolfgang Drexler
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Rainer A. Leitgeb
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Christoph K Hitzenberger
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Ronny Ramlau
    Johann Radon Institute for Computational and Applied Mathematics, Linz, Oberösterreich, Austria
  • Michael Pircher
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Footnotes
    Commercial Relationships   Elisabeth Brunner, None; Julia Shatokhina, None; Muhammad Shirazi, Imagine Eyes (F); Wolfgang Drexler, None; Rainer Leitgeb, None; Christoph Hitzenberger, None; Ronny Ramlau, None; Michael Pircher, Imagine Eyes (F)
  • Footnotes
    Support  SFB "Tomography across the scales" F6803-N36, MERLIN EU-HORIZON 2020 ICT
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 23. doi:
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      Elisabeth Brunner, Julia Shatokhina, Muhammad Faizan Shirazi, Wolfgang Drexler, Rainer A. Leitgeb, Christoph K Hitzenberger, Ronny Ramlau, Michael Pircher; AO-OCT imaging of the retina with a pyramid wavefront sensor. Invest. Ophthalmol. Vis. Sci. 2021;62(8):23.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the performance of a pyramid wavefront sensor (P-WFS) for adaptive optics (AO) imaging in visual science. The P-WFS, first introduced in astronomy, promises greater sensitivity and more flexibility than the well-established Shack-Hartmann (SH-)WFS. However, ophthalmic applications of the P-WFS have not met the performance of the SH-WFS so far.

Methods : The P-WFS was implemented as an add-on (see Fig. a)) to a SH-WFS based spectral domain AO optical coherence tomography (OCT) setup. The instrument provides an axial resolution of 4.5 μm at 840 nm central wavelength and an A-scan rate of 250 kHz. For wavefront sensing, part of the imaging light returning from the retina is used. The scanning motion of the imaging beam over the retina creates an averaging effect in the WFS data which is essential for successful AO correction with the P-WFS (see Fig. b)). Retinal images were recorded in the fovea of several healthy volunteers with the P-WFS and the SH-WFS in a single session. En-face visualization of the photoreceptor layer via segmentation and cross-sectional image data allow for a comparison of the two sensor types.

Results : The representative image data displayed in Fig. c) show en-face images of the photoreceptor mosaic and B-scan images obtained with the P-WFS and the SH-WFS. The power spectra of the enface images show the ability of the instrument to resolve the photoreceptors using either sensor type. With the P-WFS, single cone photoreceptors close to the fovea centralis can be visualized in almost the entire fovea clearly matching the image quality achieved with the SH-WFS. It is subject of on-going investigation if the clearer appearance of the photoreceptor cells and the higher clarity of the Yellott’s ring obtained for the P-WFS data is due to a general superiority of the sensor in ophthalmic applications.

Conclusions : The suitability of the P-WFS for AO imaging in visual science is demonstrated in vivo with AO-OCT. The parallel implementation of the P-WFS and a SH-WFS allows for a direct comparison of the two sensor types. Photoreceptor cells could be visualized in the fovea with both sensors demonstrating the high resolution capability of the system. The image quality obtained with the P-WFS equals or even outperforms that of the SH-WFS indicating that the P-WFS can be a promising alternative for the SH-WFS in ophthalmic imaging.

This is a 2021 ARVO Annual Meeting abstract.

 

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