June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Optimal Retinal Nerve Fiber Layer Sampling Location with OCT in Rhesus Monkeys.
Author Affiliations & Notes
  • Ronald Zambrano
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Anse Vellappally
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Palaiologos Alexopoulos
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Mengfei Wu
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Departments of Population Health and Environmental Medicine, NYU Langone Health, New York, New York, United States
  • Arturo Osvaldo Barron Arrambide
    Departments of Ophthalmology and Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, New York, United States
  • James P. Higham
    Department of Anthropology, NYU College of Arts and Sciences, New York, New York, United States
  • Amanda Dawn Melin
    Departments of Anthropology and Archaeology and Medical Genetics, University of Calgary, Calgary, Alberta, Canada
  • John Danias
    Departments of Ophthalmology and Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, New York, United States
  • Mengling Liu
    Departments of Population Health and Environmental Medicine, NYU Langone Health, New York, New York, United States
  • Joel S Schuman
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Medicine, Brooklyn, New York, United States
  • Hiroshi Ishikawa
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Medicine, Brooklyn, New York, United States
  • Gadi Wollstein
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Medicine, Brooklyn, New York, United States
  • Footnotes
    Commercial Relationships   Ronald Zambrano, None; Anse Vellappally, None; Palaiologos Alexopoulos, None; Mengfei Wu, None; Arturo Barron Arrambide, None; James Higham, None; Amanda Melin, None; John Danias, None; Mengling Liu, None; Joel Schuman, Zeiss (P); Hiroshi Ishikawa, None; Gadi Wollstein, None
  • Footnotes
    Support  NIH R01-EY030770, P30EY013079, and an unrestricted grant from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1845. doi:
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      Ronald Zambrano, Anse Vellappally, Palaiologos Alexopoulos, Mengfei Wu, Arturo Osvaldo Barron Arrambide, James P. Higham, Amanda Dawn Melin, John Danias, Mengling Liu, Joel S Schuman, Hiroshi Ishikawa, Gadi Wollstein; Optimal Retinal Nerve Fiber Layer Sampling Location with OCT in Rhesus Monkeys.. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1845.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the least variable sampling location for OCT retinal nerve fiber layer (RNFL) thickness measurements on rhesus macaque monkeys, for determining the preferred sampling location.

Methods : In vivo three-dimensional spectral-domain OCT scans (Leica, Chicago, IL) were obtained as raster scan data (400x400x1024) in a 5x5x1.6 mm region (human equivalent, not the actual size in the monkey eye) centered on the optic nerve head (ONH) of 33 healthy adult rhesus macaques (19 males, 14 females; ages 3.0-10.7 years). The ONH scans of 48 eyes were analyzed using OCT segmentation software of our own design to calculate point-by-point RNFL thickness measurements. Mean RNFL thickness was computed on consecutive concentric circles within the scan window, centered on the geometric ONH center and starting at the optic disc margin (between 64-119 circles). The least variable RNFL measurement area was identified in the vicinity of the RNFL peak within the 2 µm deviation.

Results : The least variable RNFL was observed in between 98.88±11.82 and 114.4±11.32 pixels from the ONH center with the peak RNFL at 106.42±11.55 pixels (Figure 1). Note that the number of available eyes in each sampling location varied as detailed in Figure 2. For comparison, the radius of the OCT scan circle conventionally used in humans is 1.7 mm, or 136 pixels, from the center of the ONH.

Conclusions : In order to obtain less variable circumpapillary RNFL thickness measurements on rhesus macaque monkey eyes, it is recommended to use a sampling circle with a radius of approximately 106 pixels from the ONH center, which is smaller than the human equivalent.

This is a 2021 ARVO Annual Meeting abstract.

 

 

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