June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Regional Differences of Novel Limbal Palisade of Vogt Complex Parameters using En Face Optical Coherence Tomography in Healthy Subjects
Author Affiliations & Notes
  • Daniel Saukkonen
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • William Binotti
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • Anya de Leeuw
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • Betul Bayraktutar
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • M. Cuneyt Ozmen
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • Pedram Hamrah
    Ophthalmology, Tufts Medical Center, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Daniel Saukkonen, None; William Binotti, None; Anya de Leeuw, None; Betul Bayraktutar, None; M. Cuneyt Ozmen, None; Pedram Hamrah, Heidelberg Engineering (C)
  • Footnotes
    Support  Tufts Medical Center Institutional Support; Massachusetts Lions Eye Research Fund, Inc., Research to Prevent Blindness Challenge grant to the department of Ophthalmology.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 854. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Daniel Saukkonen, William Binotti, Anya de Leeuw, Betul Bayraktutar, M. Cuneyt Ozmen, Pedram Hamrah; Regional Differences of Novel Limbal Palisade of Vogt Complex Parameters using En Face Optical Coherence Tomography in Healthy Subjects. Invest. Ophthalmol. Vis. Sci. 2021;62(8):854.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To explore the capability of en face optical coherence tomography (OCT) in measuring the dimensions of the limbal palisades of Vogt complex (PVC) and describe the regional differences in healthy subjects.

Methods : Retrospective analysis of limbal en face OCT images from 10 eyes of 10 healthy individuals. The best quality image from all available regions (nasal, temporal, superior, inferior) were selected for each subject. PVC was defined as the hyporeflective tissue within the anatomical limbus, including the palisades of Vogt and the surrounding limbal epithelium. Three-dimensional en face scans through the PVC, of 9µm depth each, were assessed to determine the anterior and superficial borders (end of Bowman’s layer) and the posterior and deep borders (transition of hyper-hyporeflective sclera-limbus). The area and volume of each scan were measured manually with ImageJ (Figure 1). Minimum and maximum depth were determined as the first and last appearance of the PVC on the OCT device, respectively. Statistical models were performed to account for different scans of the same eye and age.

Results : A total of 10 nasal, 10 temporal, 5 superior and 5 inferior en face OCTs regions were assessed. Mean age was 39.5±13.6 years [26-77] with equal male-to-female ratio. En face OCT PVC was detected in all regions. PVC area, volume, maximum area, maximum depth and thickness were greater in the superior and inferior limbus compared to the nasal and temporal (1.18±0.10 and 0.68±0.05mm2, p=0.003; 3.15±0.24 and 1.90±0.19mm2, p<0.001; 0.17±0.02 and 0.06±0.01mm3, p<0.001; 129.4±5.0 and 107.7±3.4µm, p=0.002; and 63.4±3.8 and 44.7±4.2µm, p<0.001; respectively). PVC minimum depth was not statistically significant between the regions (62.5±3.1 and 69.0±12.2µm, p=0.116; respectively). Age was not a significant covariate in this study.

Conclusions : Superior and inferior PVC area, maximum area, volume, maximum depth and thickness were greater than nasal and temporal PVC, in accordance to previous histopathological reports on regional stem cell density. En face OCT can provide novel and potentially clinical useful quantitative measurements to assess PVC health.

This is a 2021 ARVO Annual Meeting abstract.

 

Figure 1- A) Superior limbus en face OCT 3-dimensional stack. PVC area (B) and volume (C) measurement representation.

Figure 1- A) Superior limbus en face OCT 3-dimensional stack. PVC area (B) and volume (C) measurement representation.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×