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Palaiologos Alexopoulos, Behnam Tayebi, Zeinab Ghassabi, Mengfei Wu, Mengling Liu, Yoav Glidai, Katie Lucy, Bo Wang, Ian A Sigal, Matthew Smith, Anse Vellappally, Ronald Zambrano, Hiroshi Ishikawa, Joel S Schuman, Gadi Wollstein; Under pressure: Response of the lamina cribrosa pore tortuosity to acute pressure changes. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1866.
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Lamina cribrosa (LC) deformation is hypothesized to be a major cause of glaucoma. The LC undergoes different forms of stress both anteriorly from intraocular pressure (IOP), as well as posteriorly and circumferentially from subarachnoid cerebrospinal fluid pressure (CSFP) and the sclera. The purpose of this study was to determine possible in vivo changes in the path of the lamina pores under different IOP settings while maintaining fixed CSFP.
Spectral-domain OCT scans (Leica, Chicago, IL) of the optic nerve head (ONH) were acquired in vivo under different pressure settings from healthy rhesus monkeys. IOP was controlled using a gravity-based perfusion system through a needle inserted into the anterior chamber. CSFP was maintained at the baseline opening pressure via gravity-based perfusion system through cannulation of the brain’s lateral ventricle (range 8-12mmHg). Scans were acquired at baseline IOP (15mmHg), high (30 mmHg) and very high IOP (40-50 mmHg) and registered in 3D. Pores from shared regions were automatically segmented using a previously described segmentation algorithm. The path of each pore was tracked based on the calculated geometric centroid of each pore. The tortuosity of each pore path was defined as the total actual distance of the centroid path divided by the minimal distance between the first (most anterior) and last (most posterior) pore centroids.
7 eyes from 6 healthy adult Rhesus macaque were analyzed. The mean value of the pore path tortuosity varies between eyes at baseline IOP levels (range: 1.16-1.68; Table). Two main overall patterns of pore path tortuosity were detected in response to increased IOP at fixed CSFP: 4 eyes became more tortuous (M2, M5, M8, M11); in the rest of the eyes (M6 OD, M6 OS, M10) the pore paths remained either unchanged or showed a variable response. No statistically significant change (p > 0.05) was observed in this small sample in either the subject-specific analysis or the analysis of the pooled combined values of the pore path tortuosity.
Baseline pore tortuosity as well as the response of the pores to acute IOP increase varies between eyes. Further investigation is warranted to determine if these differences are associated with glaucoma susceptibility.
This is a 2021 ARVO Annual Meeting abstract.
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