June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Normative OCT optic nerve head parameters of rhesus macaques
Author Affiliations & Notes
  • Anse Vellappally
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Palaiologos Alexopoulos
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Alexi Geevarghese
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Mengfei Wu
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Population Health and Environmental Medicine, NYU Langone Health, New York, New York, United States
  • Ronald Zambrano
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Behnam Tayebi
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
  • Arturo Osvaldo Barron Arrambide
    Departments of Ophthalmology and Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, New York, United States
  • Mengling Liu
    Department of Population Health and Environmental Medicine, NYU Langone Health, New York, New York, United States
  • James P. Higham
    Department of Anthropology, NYU College of Arts and Sciences, New York, New York, United States
  • Amanda Dawn Melin
    Departments of Anthropology and Archaeology and Medical Genetics, University of Calgary, Calgary, Alberta, Canada
  • John Danias
    Departments of Ophthalmology and Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, New York, United States
  • Joel S Schuman
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, United States
  • Hiroshi Ishikawa
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, United States
  • Gadi Wollstein
    Department of Ophthalmology, NYU Langone Health, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, United States
  • Footnotes
    Commercial Relationships   Anse Vellappally, None; Palaiologos Alexopoulos, None; Alexi Geevarghese, None; Mengfei Wu, None; Ronald Zambrano, None; Behnam Tayebi, None; Arturo Barron Arrambide, None; Mengling Liu, None; James Higham, None; Amanda Melin, None; John Danias, None; Joel Schuman, Zeiss (P); Hiroshi Ishikawa, None; Gadi Wollstein, None
  • Footnotes
    Support  NIH R01-EY030770 and unrestricted grant from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1848. doi:
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      Anse Vellappally, Palaiologos Alexopoulos, Alexi Geevarghese, Mengfei Wu, Ronald Zambrano, Behnam Tayebi, Arturo Osvaldo Barron Arrambide, Mengling Liu, James P. Higham, Amanda Dawn Melin, John Danias, Joel S Schuman, Hiroshi Ishikawa, Gadi Wollstein; Normative OCT optic nerve head parameters of rhesus macaques. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1848.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Rhesus macaques are a common animal model in ophthalmology because of the high similarity of their eyes and visual pathway to human. The characterization of optic nerve head (ONH) and peripapillary region in monkeys reported so far mostly involved a manual process which is laborious and subjected to operator errors. It is also usually generated from a cohort of similar age group. In this cross-sectional observational study, we deploy automated and manual segmentations to evaluate the OCT retinal nerve fiber layer (RNFL) thickness, ONH and lamina cribrosa (LC) microstructure parameters in a cohort of free roaming macaques.

Methods : In-vivo ONH spectral-domain OCT scans (Leica, Chicago, IL) were obtained by a single experienced operator after excluding eyes with any retinal pathologies. The margins of the optic disc were drawn manually and the resultant scans were analyzed using an automated segmentation software of our own design. The LC microstructure parameters were obtained through a previously described segmentation algorithm. The other parameters of ONH, namely the cup-to-disc (C/D) ratio and minimum rim width (MRW) were assessed manually. Wilcoxon rank sum test was used to test the association of LC parameters, C/D ratio and MRW with age, while the rest of the parameters were analyzed using mixed effects model accounting for age, sex and intra-subject correlation.

Results : 29 eyes from 19 monkeys (11 females, 8 males) with age ranging from 4.2 to 23.8 years were analyzed. Males were overall bigger and significantly heavier than females in our cohort (Table 1). Superior RNFL was thicker in male and is the only RNFL parameter that was associated with age or sex in this healthy cohort. No significant association was detected for any of the ONH parameters with age or sex. LC was more visible and thicker in male with higher beam to pore ratio and connective tissue fraction than in female.

Conclusions : The characterization of normal macaque eyes from a cohort of free roaming animals is useful as a standard reference to assess pathological changes in future experimental studies.

This is a 2021 ARVO Annual Meeting abstract.

 

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