June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Longitudinal retinal image analysis of diabetic Akita mice
Author Affiliations & Notes
  • Rupesh Singh
    Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Mariya Ali
    Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Julia C Batoki
    Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Bela Anand-Apte
    Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
    Cleveland Clinic Lerner Research Institute, Cleveland, Ohio, United States
  • Footnotes
    Commercial Relationships   Rupesh Singh, None; Mariya Ali, None; Julia Batoki, None; Bela Anand-Apte, None
  • Footnotes
    Support  NIH-NEI P30 Core Grant (IP30EY025585), Unrestricted Grants from The Research to Prevent Blindness, Inc., and Cleveland Eye Bank Foundation awarded to the Cole Eye Institute.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1104. doi:
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      Rupesh Singh, Mariya Ali, Julia C Batoki, Bela Anand-Apte; Longitudinal retinal image analysis of diabetic Akita mice. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1104.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To evaluate the changes in the retinal structure and retinal vasculature in type-1 diabetic mice (C57BL/6-Ins2Akita/J ) over time.

Methods : Fluorescein angiography (FA), Indocyanine green angiography (ICGA) and OCT imaging was performed on Akita mice at 3 months and 6 months of age. The confocal scanning laser ophthalmoscopy (cSLO) images were analyzed for FA and ICGA. OCT images were evaluated for thickness measurement of distinct layers of the retina. A total of 25 (male) animals were used in the study. FA was performed after IP injection of Na- fluorescein, and images were collected at ~three mins and ~six mins for early and late phase of dye circulation. ICG was administered via tail vein. Statistical comparison were made using non-parametric two tailed t-test with significance at P<0.05.

Results : Using ImageJ, a simple thresholding protocol was developed, which uses IsoData algorithm, and fixed lower and upper threshold values for all the FA and ICG images. Both FA and ICGA showed significantly increased retinal vasculature leakage (92% increase in FA and 101.9 % increase in ICGA) in 6-month old Akita mice compared with age matched WT (Fig. 1). OCT based total retinal thickness was significantly reduced in Akita mice at both three (2.8%) and six (2.5%) months of age. The middle retinal layer thicknesses, OPL and ONL were significantly different in mice in the 6-month age group (Fig.2). However, at three months, only the OPL layer had thinning in the outer and middle retinal region (Fig. 2). No observable changes were observed in the outer retinal layers (IS/OS and RPE).

Conclusions : Aging diabetic mice exhibit clear imaging biomarkers such as retinal fluid leakage and inner retinal layer degeneration.

This is a 2021 ARVO Annual Meeting abstract.




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