June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
EFFICACY of CELL-BASED PLASMACYTOID DENDRITIC CELL THERAPY FOR CORNEAL WOUND HEALING
Author Affiliations & Notes
  • Arsia Jamali
    Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
    Cornea Service, New England Eye Center, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Betul Bayraktutar
    Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
    Cornea Service, New England Eye Center, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Deshea L Harris
    Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Victor G. Sendra
    Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Pedram Hamrah
    Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
    Cornea Service, New England Eye Center, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Arsia Jamali, None; Betul Bayraktutar, None; Deshea Harris, None; Victor Sendra, None; Pedram Hamrah, None
  • Footnotes
    Support  NIH-R01-EY022695 (PH), R01-EY029602 (PH), NIH-R21-EY025393 (PH). Tufts Institutional Support (PH), Research to Prevent Blindness Challenge Grant to the Department of Ophthalmology.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 903. doi:
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      Arsia Jamali, Betul Bayraktutar, Deshea L Harris, Victor G. Sendra, Pedram Hamrah; EFFICACY of CELL-BASED PLASMACYTOID DENDRITIC CELL THERAPY FOR CORNEAL WOUND HEALING. Invest. Ophthalmol. Vis. Sci. 2021;62(8):903.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have recently shown that plasmacytoid dendritic cells (pDCs) play a significant role in corneal homeostasis. The current study aims to determine the efficacy and safety of local adoptive transfer of pDCs for the treatment of corneal epithelial wounds.

Methods : Splenic pDCs from 6-8-week-old wild-type (WT) C57BL/6 or DPE-GFP×RAG1-/- mice, receiving FLT3L-secreting B16 melanoma cells to enhance pDC density, were isolated by flow cytometric sorting. Central corneal epithelial debridement using a 2 mm trephine was performed on WT mice to assess the density of labelled pDCs. Epithelial debridement of central corneas was performed on PSGL-1 KO mice using a 1.5 mm trephine in order to assess the effect of pDC therapy on corneal wounds, ruling out the effect of infiltrating cells. Mice received topical fibrin sealant (sham-transfer) or 10,000 splenic pDCs. Corneal fluorescein staining was conducted 24, 48, and 72 hours after the procedure to assess wound healing. Wound areas were measured using ImageJ. Corneal would-mounts were imaged on day 2, 5, 7, and 14 after the adoptive transfer to measure the density of transferred cells. T test was used to determine differences and p<0.05 was considered significant.

Results : Adoptive transfer did not result in corneal opacity or neovascularization. Adoptively-transferred GFP+ pDCs were observed in the central cornea on day 2 and remained in the cornea 14 days following adoptive transfer, although in reduced numbers compared to day 2 (p<0.05). We observed that one-time adoptive transfer of pDCs significantly decreased epithelial defect size by 1.7-, 3.0- and 6.1-fold, at 24, 48, and 72 hour following corneal epithelial wounding, as compared to sham-transfer (p<0.05).

Conclusions : Local adoptive transfer of pDCs is a safe procedure, which significantly improves corneal wound healing.

This is a 2021 ARVO Annual Meeting abstract.

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