June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Rheological and Proteomics Characterization of Thermoresponsive Hydrogel from Porcine Cornea Extracellular Matrix for Corneal Tissue Engineering Purposes
Author Affiliations & Notes
  • Ghasem Yazdanpanah
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
    Richard and Loan Hill Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States
  • Yizhou Jiang
    Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, Chicago, Illinois, United States
  • Sayena Jabbehdari
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Khandaker Anwar
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Xiang Shen
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Seungwon An
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Elmira Jalilian
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Meisam Omidi
    Marquette University School of Dentistry, Milwaukee, Wisconsin, United States
  • Mark Rosenblatt
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Tolou Shokuhfar
    Richard and Loan Hill Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States
  • Yayue Pan
    Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, Chicago, Illinois, United States
  • Alexandra Naba
    Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois, United States
  • Ali R Djalilian
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Ghasem Yazdanpanah, None; Yizhou Jiang, None; Sayena Jabbehdari, None; Khandaker Anwar, None; Xiang Shen, None; Seungwon An, None; Elmira Jalilian, None; Meisam Omidi, None; Mark Rosenblatt, None; Tolou Shokuhfar, None; Yayue Pan, None; Alexandra Naba, None; Ali Djalilian, None
  • Footnotes
    Support  This work was supported by R01 EY024349 (ARD), Core Grant for Vision Research EY01792 (MIR) from NEI/NIH; Unrestricted Grant to the Department and Physician-Scientist Award both from Research to Prevent Blindness; Eversight; and the Searle Funds at the Chicago Community Trust (AN). Proteomics services were provided by the UIC Research Resources Center Mass spectrometry Core which was established in part by a grant from The Searle Funds at the Chicago Community Trust to the Chicago Biomedical Consortium. Bioinformatic analyses were performed by the UIC Research Informatics Core, supported in part by the National Center for Advancing Translational Sciences (NCATS, Grant UL1TR002003).
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 872. doi:
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      Ghasem Yazdanpanah, Yizhou Jiang, Sayena Jabbehdari, Khandaker Anwar, Xiang Shen, Seungwon An, Elmira Jalilian, Meisam Omidi, Mark Rosenblatt, Tolou Shokuhfar, Yayue Pan, Alexandra Naba, Ali R Djalilian; Rheological and Proteomics Characterization of Thermoresponsive Hydrogel from Porcine Cornea Extracellular Matrix for Corneal Tissue Engineering Purposes. Invest. Ophthalmol. Vis. Sci. 2021;62(8):872.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Fabricating thermoresponsive hydrogels from decellularized tissues is a trending approach to develop novel therapeutic biomaterials for tissue engineering purposes. There are differences in the characteristics of the produced hydrogel related to the source of the tissue as well as the decellularization and solubilization protocols. Detailed characterization of the hydrogels will support the efforts to optimize their application as biomaterials for tissue engineering and therapeutics. Here, we fabricated an in-situ thermoresponsive hydrogel from decellularized porcine cornea extracellular matrix, COMatrix (COrnea Matrix), and characterized its structure and thermoresponsive rheological behavior (heat-induced Sol-Gel transition) as well as exploring its protein composition using proteomic approaches.

Methods : The COMatrix hydrogel was fabricated by decellularization and partial digestion of porcine cornea. The gelation was induced by mild-heat (37°C). The turbidimetric gelation kinetics was evaluated by pre-warmed spectrophotometer. The rheological gelation kinetics and properties of COMatrix was evaluated with a temperature controlled rheometer. The protein composition of COMatrix was mapped using mass spectrometric proteomics.

Results : COMatrix forms a transparent gel (10 minutes gelation time) after in-situ curing with heat, characterized by alteration in light absorbance and rheological indexes. . The highest elastic shear modulus (G’) and highest viscous modulus (G”) for 25 mg/ml hydrogels were 83.3±4.2 Pa and 12.9±7.2 Pa for 25 mg/ml samples. The rheological characterization of heat-formed COMatrix gel shows similar behavior to common biomaterials utilized in tissue engineering. The fibrillar structure of COMatrix gel was observed by scanning electron microscopy as the density of fibers attenuates in lower concentrations. Mass spectrometric proteomic analysis revealed that COMatrix hydrogel is rich in extracellular matrix proteins with known regenerative properties such as lumican, keratocan and laminin in addition to structural collagen proteins.

Conclusions : COMatrix hydrogel is a naturally driven biomaterial with favorable biomechanical properties and protein content with potential application as a therapeutic biomaterial in ocular regeneration and tissue engineering.

This is a 2021 ARVO Annual Meeting abstract.

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