June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Long-term Survival of Cultivated Oral Mucosal Epithelial Cell Sheets in post-COMET Patients
Author Affiliations & Notes
  • Yi-Jen Hsueh
    Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Hung-Chi Chen
    Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Hui-Kang David Ma
    Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Footnotes
    Commercial Relationships   Yi-Jen Hsueh, None; Hung-Chi Chen, None; Hui-Kang Ma, None
  • Footnotes
    Support  MOST grants 107-2314-B-182A-122 and 108-2314-B-182A-114
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 855. doi:
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      Yi-Jen Hsueh, Hung-Chi Chen, Hui-Kang David Ma; Long-term Survival of Cultivated Oral Mucosal Epithelial Cell Sheets in post-COMET Patients. Invest. Ophthalmol. Vis. Sci. 2021;62(8):855.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The purpose of this study is to verify that collagenase-based spheroidal suspension culture is a promising technique for cultivated oral mucosal epithelial transplantation (COMET).

Methods : Patients with limbal stem cell deficiency who received COMET to promote wound healing were studied retrospectively. Immunoconfocal microscopy was performed on corneal specimens from the patients after COMET, as well on normal corneas, conjunctiva, and oral mucosa for Keratin 3, 4, 13, p63 and p75NTR.

Results : A biopsy taken two years after COMET showed stratified epithelium with small, compact basal epithelial cells. The epithelium was positive for Keratin 4, 13, and 3 in the suprabasal layer but universally negative in the basal layer. Staining for p63 and p75NTR were both positive in the basal layer. The graft remained clear up to post-OP 4 years, and his best corrected vision remained above 20/120.

Conclusions : Both clinical observations and histological examination confirmed the long-term survival of the transplanted OMECs, suggesting that collagenase-based spheroidal suspension culture is a promising technique for COMET.

This is a 2021 ARVO Annual Meeting abstract.

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