June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Deep neural network for the analysis of guttae via semi-supervised learning in a Fuchs endothelial corneal dystrophy mouse model
Author Affiliations & Notes
  • Takeru Nishikawa
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Naoki Okumura
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Kaito Narimoto
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Shohei Yamada
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Kengo Okamura
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Ayaka Izumi
    ActualEyes Inc., Kyotanabe, Japan
  • Noriko Koizumi
    Department of Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • Footnotes
    Commercial Relationships   Takeru Nishikawa, None; Naoki Okumura, ActualEyes, Inc. (I), Doshisha University (P), Kowa Company Ltd. (C), Senju Pharmaceutical Co.,Ltd. (P); Kaito Narimoto, None; Shohei Yamada, None; Kengo Okamura, None; Ayaka Izumi, ActualEyes Inc. (E); Noriko Koizumi, ActualEyes, Inc. (I), ActualEyes, Inc. (F), Doshisha University (P), Japan Innovative Therapeutics, Inc. (F), Kowa Company Ltd. (F), Kowa Company Ltd. (C), M's Science Corporation (F), M's Science Corporation (C), Senju Pharmaceutical Co.,Ltd. (F), Senju Pharmaceutical Co.,Ltd. (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 826. doi:
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      Takeru Nishikawa, Naoki Okumura, Kaito Narimoto, Shohei Yamada, Kengo Okamura, Ayaka Izumi, Noriko Koizumi; Deep neural network for the analysis of guttae via semi-supervised learning in a Fuchs endothelial corneal dystrophy mouse model. Invest. Ophthalmol. Vis. Sci. 2021;62(8):826.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Although numerous numbers of accurately annotated data as grand truth is necessary for deep learning, the preparation of grand truth can create a bottleneck. In this study, we prepared a small number of annotated corneal endothelial images in a early-stage Fuchs endothelial corneal dystrophy (FECD) model mouse, and tested the feasibility of semi-supervised learning to widen the indication of AI to late-stage FECD.

Methods : Corneal endothelial images were obtained from FECD mouse-model eyes via contact specular microscopy. A trained model (AI 1) was created via the use of 28 manually annotated images (ground truth) of early-stage FECD mouse eyes. Supervised data 1 (n=250) of late-stage FECD was generated via AI 1, and AI 2 was generated via the use of supervised data 1. Then, supervised data 2 was generated by AI 2, and AI 3 was generated by using supervised data 2. Subsequently, those learning processes were repeated up to AI 12. Finally, AI was used to analyze the 25 test data images of late-stage FECD.

Results : AI 1 was generated via the 28 annotated image data of early-stage FECD. The guttae area detected by AI 1 was strongly associated with ground truth in early-stage FECD (r=0.97, p=3.83×10-27), however, AI 1 underestimated the guttae area by a mean systematic error (i.e., between the guttae area detected by AI and ground truth) of -2.2 ± 1.3% (r=0.86, p=2.83×10-8) in late-stage FECD. After semi-supervised learning, systematic error tended to decrease throughout AI 2-9, though it increased slightly due to overestimation in AI 10-12. The mean systematic error of AI 9 was -0.1±0.9%, and the guttae area detected by AI 9 was strongly associated with the manually annotated test data of late-stage FECD (r=0.94, p=5.84×10-12).

Conclusions : Semi-supervised learning by the use of limited numbers of annotated data of early-stage FECD enables the generation of a deep neural network for analyzing different disease phases of late-stage FECD. Our data suggests that semi-supervised learning can be applicable to multiple clinical settings; e.g., reduction of the time and cost of preparing annotated data and expanded indication (to very early or late stages) of a deep neural network.

This is a 2021 ARVO Annual Meeting abstract.

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