June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Histatin -5 inhibits expression of pro-inflammatory cytokines through TLR- NF-kappa B pathway induced by hyperosmolar conditions in human corneal epithelial cells
Author Affiliations & Notes
  • Dhara Shah
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Kyung-No Son
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Sang Min Lee
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Marwan Ali
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Arun Balasubramaniam
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Vinay Kumar Aakalu
    Illinois Eye and Ear Infirmary, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Dhara Shah, None; Kyung-No Son, None; Sang Min Lee, None; Marwan Ali, None; Arun Balasubramaniam, None; Vinay Aakalu, Horizon Pharma (C), University of Illinois at Chicago (P), ViSo Therapeutics Inc. (I)
  • Footnotes
    Support  NEI/NIH K08EY024339, R01EY029409, Department of Defense:W81XWH1710122, VA I01BX004080, Unrestricted Grant, Research to Prevent Blindness NEI P30EY001792
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 698. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Dhara Shah, Kyung-No Son, Sang Min Lee, Marwan Ali, Arun Balasubramaniam, Vinay Kumar Aakalu; Histatin -5 inhibits expression of pro-inflammatory cytokines through TLR- NF-kappa B pathway induced by hyperosmolar conditions in human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2021;62(8):698.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Toll-like receptors (TLRs) have a key role in triggering the innate immune response. TLR activation causes the production of various pro-inflammatory cytokines, chemokines, and antimicrobial peptides (AMPs) commonly found on the ocular surface. Histatin peptides are potent anti-microbial agents that may have salubrious effects in ocular epithelia. Multiple ocular surface diseases (OSD) including dry eye disease (DED), are driven by inflammation. Here, we investigated how histatin 5 (Hst5) modulates the expression of TLRs in an in vitro experimental model of DED.

Methods : Hyperosmolarity was induced by adding 125 mM NaCl with or without co-treatment with Hst5 in human corneal epithelial (HCE) cells. Cell viability was measured using WST-assay kit following the manufacturer’s instruction. The expression of TLRs (TLR2, TLR3, TLR4 and TLR7) and pro-inflammatory cytokines (IL-6, IL-8 and TNF-α) were examined by qPCR. NF-κB activation was examined by detecting IκB-α degradation using Western blotting and p65 translocation to nucleus using immunofluorescence staining.

Results : Experimental results showed that Hst5 abrogated cell death induced by hyperosmolar condition in HCE cells. Hyperosmolar conditions significantly induced TLR2 and TLR7 in HCE cells. TLR signaling was inhibited by co-treatment of Hst5. Application of Hst5 reduced the synthesis ocular surface disease relevant pro-inflammatory cytokines significantly. We also found that activation of NF-kB was reduced in response to Hst5.

Conclusions : Thus, Hst5 application can reduce hyperosmolarity related TLR signaling and innate immune inflammation in the ocular surface.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×