June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Evaluation of Antiviral Efficacy for Contact Lens Care Products with Herpes Simplex Virus
Author Affiliations & Notes
  • Cynthia McAnally
    R&D Microbiology, Alcon Laboratories Inc, Fort Worth, Texas, United States
  • Rhonda Walters
    R&D Microbiology, Alcon Laboratories Inc, Fort Worth, Texas, United States
  • Manal M Gabriel
    R&D Microbiology, Alcon Laboratories Inc, Fort Worth, Texas, United States
  • Paul Shannon
    R&D Microbiology, Alcon Laboratories Inc, Fort Worth, Texas, United States
  • Footnotes
    Commercial Relationships   Cynthia McAnally, Alcon Laboratories (E); Rhonda Walters, Alcon Laboratories (E); Manal Gabriel, Alcon Laboratories (E); Paul Shannon, Alcon Laboratories (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 677. doi:
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    • Get Citation

      Cynthia McAnally, Rhonda Walters, Manal M Gabriel, Paul Shannon; Evaluation of Antiviral Efficacy for Contact Lens Care Products with Herpes Simplex Virus. Invest. Ophthalmol. Vis. Sci. 2021;62(8):677.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Contact lens care (CLC) products require robust activity against microorganisms in order to clean and disinfect contact lenses (CL). CLC products are required to meet criteria for disinfection of CLs against bacteria, yeast and mold. However, contamination of CLs is not limited to microorganisms, as viruses can also cause keratitis in patients. Herpes simplex virus type 1 (HSV-1) is the leading infectious cause of keratitis and reoccurrences are common. This study assesses multipurpose and hydrogen peroxide CLC products for antiviral efficacy against HSV-1 in the presence and absence of contact lenses.

Methods : A modified version of ISO 14729 was utilized to assess the antiviral activity of CLC products against Human herpes virus 1 ATCC VR 260 with stand-alone and/or regimen testing. Three multipurpose solutions containing polyquaternium-1 and myristamidopropyl dimethylamine (MPS-1,-2,-3), and two hydrogen peroxide products containing 3% hydrogen peroxide (HP-1,-2) were utilized. For stand-alone tests, all three MPS were inoculated with 105-108 TCID50/mL of HSV-1 and allowed to disinfect for six hours prior to recovery of viable virus particles. For regimen tests with MPS-1, HP-1 and HP-2, soft contact lenses (Groups 1 and 4) were inoculated with 105-107 TCID50/lens, cleaned and/or rinsed, and soaked in lens cases according to label instructions. Following disinfection time, samples were serially diluted and plated on VERO cells in order to determine the titer of viable virus. Log and percent (%) reductions were determined based differences between starting counts and survivors at DT. There are no criteria for the antiviral efficacy of CLC products tested with Herpes simplex virus.

Results : For MPS-1, MPS-2 and MPS-3, the log and percent reduction in the stand-alone test (without lenses) at disinfection time was 3.2 (99.9%), 1.7 (90%) and 1.5 (90%) respectively. For the regimen test (with lenses in lens cases), no viable virus particles were recovered from the lenses or from MPS-1, HP-1 or HP-2 solutions after completing the cleaning, and/or rinsing, and disinfection steps resulting in an avg. log reduction of 5.0 (99.999%).

Conclusions : Contact lens care products tested with and without contact lenses showed antiviral activity in tests for Human herpesvirus 1. This information could be useful when counseling contact lens patients with a history of HSV-1 keratitis.

This is a 2021 ARVO Annual Meeting abstract.

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