June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Pulsed Low Frequency Ultrasound for Corneal Phonophoresis
Author Affiliations & Notes
  • Ricardo Lamy
    Ophthalmology, University of California San Francisco, San Francisco, California, United States
  • Peter D Jones
    Radiation Oncology, University of California San Francisco, San Francisco, California, United States
  • Manjiri Sahasrabudhe
    Ophthalmology, University of California San Francisco, San Francisco, California, United States
  • Chris J Diederich
    Radiation Oncology, University of California San Francisco, San Francisco, California, United States
  • Jay M Stewart
    Ophthalmology, University of California San Francisco, San Francisco, California, United States
  • Footnotes
    Commercial Relationships   Ricardo Lamy, None; Peter Jones, None; Manjiri Sahasrabudhe, None; Chris Diederich, None; Jay Stewart, None
  • Footnotes
    Support   NIH 1R01EY024004; NIH Core Grant for Vision Research EY002162; Research to Prevent Blindness, Inc., New York, NY; That Man May See, Inc., San Francisco, CA.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1210. doi:
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    • Get Citation

      Ricardo Lamy, Peter D Jones, Manjiri Sahasrabudhe, Chris J Diederich, Jay M Stewart; Pulsed Low Frequency Ultrasound for Corneal Phonophoresis. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1210.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Purpose : To determine the effect of pulsed low frequency ultrasound (PLFU) at 250 kHz and 500 kHz on the delivery of topical compounds into the corneal stroma.

Methods : Fresh cadaveric rabbit eyes with intact corneal epithelium were placed in a solution of 0.1% riboflavin. Treated eyes received PLFU (60% duty cycle) with a Spatial Average Temporal Average Intensity (Isata) of 1.0 W/cm2 for 6 minutes at either 250 kHz or 500 kHz, and the eyes were then left in riboflavin solution for a total immersion time of 20 minutes. Control eyes without ultrasound treatment received the same exposure to riboflavin solution. Corneas were then excised, and confocal microscopy was performed to measure the penetration and distribution of riboflavin in the corneal stroma.

Results : At a corneal depth of 300 microns, the average fluorescence intensity of riboflavin in the groups treated with PLFU 250 kHz and 500 kHz was respectively 9232 ± 2595 A.U. (n = 6) and 7454 ± 2184 A.U. (n=6). When compared to controls (1449 ± 690 A.U.; n=6) the difference was statistically significant for both treated groups (p-value < 0.05), demonstrating 5 - 6 fold therapeutic gain. The difference between corneas treated at 250 KHz and 500 KHz was not statistically significant.

Conclusions : PLFU was very effective in delivering riboflavin into the corneal stroma despite the presence of a previously intact epithelial barrier. This approach may offer a means of achieving clinically useful concentrations and penetration of topically applied drugs in the corneal stroma without removing the corneal epithelium.

This is a 2021 ARVO Annual Meeting abstract.

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