June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Phase resolved OCT to measure laser induced refractive index change (LIRIC) in hydrogel
Author Affiliations & Notes
  • Aaron Michalko
    Clerio Vision, Inc., Rochester, New York, United States
  • Vimal Prabhu Pandiyan
    Ophthalmology, University of Washington, Seattle, Washington, United States
  • Ramkumar Sabesan
    Ophthalmology, University of Washington, Seattle, Washington, United States
  • Len Zheleznyak
    Clerio Vision, Inc., Rochester, New York, United States
  • Footnotes
    Commercial Relationships   Aaron Michalko, Clerio Vision, Inc. (E); Vimal Prabhu Pandiyan, University of Washington (P); Ramkumar Sabesan, University of Washington (P); Len Zheleznyak, Clerio Vision, Inc. (E)
  • Footnotes
    Support  NIH U01EY025501, R21EY027941, R01EY029710, P30EY001730, Unrestricted grant from the Research to Prevent Blindness, Research to Prevent Blindness Career Development Award, Burroughs Welcome Fund Careers at the Scientific Interfaces, Murdock Charitable Trust
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1813. doi:
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    • Get Citation

      Aaron Michalko, Vimal Prabhu Pandiyan, Ramkumar Sabesan, Len Zheleznyak; Phase resolved OCT to measure laser induced refractive index change (LIRIC) in hydrogel. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1813.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : LIRIC is a non-invasive method for correcting aberrations in hydrogels, IOLs and the cornea. As such, the need exists for non-destructive metrology of LIRIC-induced optical phase change. Here we demonstrate the feasibility and validation of parallel phase resolved OCT for measurements of optical phase change induced by LIRIC in hydrogel.

Methods : To produce the LIRIC sample, a femtosecond laser was focused inside a 0.5 mm thick hydrogel button (Contamac, Acofilcon A) and scanned throughout a single layer. Seven rectangular regions (0.1 x 0.4 mm each) of LIRIC piston wavefronts were induced. Each region was written at a distinct laser power (0.7 to 1.0 W, in 0.05 W steps). The LIRIC-induced phase shift was subsequently measured in two methods: (a) phase-resolved line-scan OCT and (b) phase-shifting interferometry (PSI). The line-scan, spectral domain OCT (λ=820 nm, Δλ=80 nm) was used to image a 3.5x2.5 mm field-of-view on the hydrogel. The 3D phase distribution was obtained in the reconstructed complex volume after segmenting the LIRIC layer in depth. The PSI consisted of a Mach-Zehnder interferometer (λ=632 nm) with a piezo-driven phase-shifting mirror in the reference arm. LIRIC was quantified from both OCT and PSI by computing the difference in optical phase between written and unwritten regions of the hydrogel.

Results : The intended spatial profile of the LIRIC-induced wavefront was readily reproduced in both measurements. Both measurement techniques (OCT and PSI) showed a predictable increase in the magnitude of phase change with increasing laser power. With OCT, it ranged from 76±58 to 376±91 nm at 0.7 and 1.0 W, respectively. With PSI, it ranged from 61±29 to 334±40 nm. The OCT and PSI measurements were highly correlated (R-squared = 0.99).

Conclusions : Phase resolved OCT was shown to accurately measure optical phase change in hydrogels induced via femtosecond laser processing (LIRIC).

This is a 2021 ARVO Annual Meeting abstract.

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