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YOICHIRO SHINKAI, Kentaro Kojima, Hiroshi Tanaka, Kazuki Kuroda, Shinichiro Ichise, Hiroshi Nose, Takaki Koide, Chie Sotozono; Evaluation of Müller cell migration on artificial collagen-like polypeptides with various functional amino-acid sequences. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1680.
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© ARVO (1962-2015); The Authors (2016-present)
Collagen, an abundantly distributed extracellular matrix protein, is known to maintain the structural integrity of organs and tissues in mammals, and it not only plays a physical function as a structural protein, but also has various biological functions that regulate cell adhesion, migration, and differentiation. In this study, we investigated the migration activity of cells in the human Müller cell line MIO-M1 with certain amino acid sequences displayed on the collagen triple helices, which can control cell adhesion, proliferation, and survival by arbitrarily incorporating collagen-derived functional amino-acid sequences.
The wells of 48-well plates were coated with a peptide polymer containing four kinds of functional amino-acid sequences (GFOGER, GLOGEN, GVXGFO, and KGHRGF) at the concentrations of 1, 3, 10, 30, and 100%, and 3mm RTV silicones were placed in the center of each well. MIO-M1 cell-line cells (5 x 104 cells/well) were then seeded in the periphery, and the silicones were removed when the cells reached 80% confluence. Photographs were taken immediately after removal and 72 hours later, the cell migration area was measured with ImageJ software, and the migration activity was then compared and evaluated to see if the migration area of the control (0%) was 1. Migration measurements were performed (n=5), and the mean value was calculated.
In the GFOGER group, the migration activity was higher in the concentration of 10, 30, and 100% compared to the control, respectively (p<0.001, unpaired t test), as well as in the concentration of 3% (p<0.01, unpaired t test). In the GLOGEN and KGHRGF groups, the migration activity was higher in the concentration of 30% and 100% compared to the control (p<0.01, unpaired t test). In the GVXGFO group, the migration activity was higher in the concentration of 100% compared to the control (p<0.01, unpaired t test).
The findings in this study showed that collagen-derived functional amino-acid sequences improved the migration activity of MIO-M1 cell-line cells.
This is a 2021 ARVO Annual Meeting abstract.
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