June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Quantitative evaluation of transplanted and host retinal tissues by optical coherence tomography
Anderson N Vu; Vidisha Marwaha; Bin Lin; Josiah K To; Bryce McLelland; Gabriel Nistor; Hans Keirstead; Igor Nasonkin; Andrew W Browne; Magdalene J Seiler
Author Affiliations & Notes
  • Anderson N Vu
    Gavin Herbert Eye Institute, Department of Ophthalmology, University of California Irvine School of Medicine, Irvine, California, United States
  • Vidisha Marwaha
    University of California San Diego, La Jolla, California, United States
    Gavin Herbert Eye Institute, Department of Ophthalmology, University of California Irvine School of Medicine, Irvine, California, United States
  • Bin Lin
    University of California Irvine Sue and Bill Gross Stem Cell Research Center, Irvine, California, United States
  • Josiah K To
    Gavin Herbert Eye Institute, Department of Ophthalmology, University of California Irvine School of Medicine, Irvine, California, United States
  • Bryce McLelland
    AIVITA Biomedical Inc., Irvine, California, United States
  • Gabriel Nistor
    AIVITA Biomedical Inc., Irvine, California, United States
  • Hans Keirstead
    AIVITA Biomedical Inc., Irvine, California, United States
  • Igor Nasonkin
    AIVITA Biomedical Inc., Irvine, California, United States
  • Andrew W Browne
    Gavin Herbert Eye Institute, Department of Ophthalmology, University of California Irvine School of Medicine, Irvine, California, United States
    Department of Biomedical Engineering, Institute for Clinical and Translational Science, University of California Irvine, Irvine, California, United States
  • Magdalene J Seiler
    University of California Irvine Sue and Bill Gross Stem Cell Research Center, Irvine, California, United States
    Department of Physical Medicine & Rehabilitation, Anatomy & Neurobiology, Ophthalmology, University of California Irvine, Irvine, California, United States
  • Footnotes
    Commercial Relationships   Anderson Vu, None; Vidisha Marwaha, None; Bin Lin, None; Josiah To, None; Bryce McLelland, AIVITA Biomedical Inc. (E); Gabriel Nistor, AIVITA Biomedical Inc. (E); Hans Keirstead, AIVITA Biomedical Inc. (E); Igor Nasonkin, AIVITA Biomedical Inc. (E); Andrew Browne, None; Magdalene Seiler, Ocular Transplantation, LLC (P)
  • Footnotes
    Support  UCI ICTS NIH KL2 Grant number is KL2 TR001416 (AB), California Institute of Regenerative Medicine (CIRM) grant number TRAN1-10995 (MS); 1R44EY027654-01A1 (IN), RPB unrestricted grant to UCI Department of Ophthalmology.
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 1676. doi:
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      Anderson N Vu, Vidisha Marwaha, Bin Lin, Josiah K To, Bryce McLelland, Gabriel Nistor, Hans Keirstead, Igor Nasonkin, Andrew W Browne, Magdalene J Seiler; Quantitative evaluation of transplanted and host retinal tissues by optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2021;62(8):1676.

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Abstract

Purpose : Retinal organoids show great promise as a transplantable treatment for retinal degeneration. Quantifying organoid anatomical features and visual function recovery in vivo after organoid transplantation involves spectral domain optical coherence tomography (SD-OCT) and superior colliculus recordings (SCR). Volumetric analysis of organoid structures and its role in visual recovery has not been investigated. We sought to correlate transplant imaging characteristics on SD-OCT with SCR functional recordings in a model of retinitis pigmentosa with rhodopsin mutation (S334ter-3).

Methods : Human embryonic stem cells (hESC) were induced into retinal organoids and differentiated in vitro for 30-70 days. Retinal organoids suitable for transplantation were chosen based on outer tissue transparency and morphological criteria. Organoids were then transplanted into the subretinal space of S334ter-3 rats. SD-OCT were completed at 2 weeks and 1 month after transplantation, then every 1-2 months. After overnight dark adaptation, extracellular recording was utilized in the superior colliculus of the rats and the data was analyzed using MATLAB according to established procedures. SD-OCT were analyzed for native retina and transplant volume, morphology, and transplant distance to the optic disc using 3D Slicer and ImageJ, both open-source image analysis software.

Results : Preliminary data demonstrated rats with positive SCR had greater final organoid volumes than rats with no SCR. Further analysis is ongoing.

Conclusions : Our data showed that lower organoid transplant volumes were linked to lower visual function recovery. This pilot study suggests that quantitative and volumetric analysis of retinal organoid transplants using SD-OCT may predict the quality of retinal transplantation.

This is a 2021 ARVO Annual Meeting abstract.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2024 Association for Research in Vision and Ophthalmology.
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