June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Microglial activation in healthy retina and experimental glaucoma during aging.
Author Affiliations & Notes
  • Ana Ramirez
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Óptica y Optometría. Departamento de Inmunología, Oftalmología y ORL. UCM, Madrid, Madrid, Spain
  • Jose A. Fernández-Albarral
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Medicina. Departamento de Inmunología, Oftalmología y ORL, Madrid, Madrid, Spain
  • Inés López Cuenca
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Medicina. Departamento de Inmunología, Oftalmología y ORL, Madrid, Madrid, Spain
  • Elena Salobrar-García
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Óptica y Optometría. Departamento de Inmunología, Oftalmología y ORL. UCM, Madrid, Madrid, Spain
  • Pilar Rojas
    Hospital General Universitario Gregorio Marañón, Instituto Oftálmico de Madrid, Madrid, Madrid, Spain
  • Rosa de Hoz
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Óptica y Optometría. Departamento de Inmunología, Oftalmología y ORL. UCM, Madrid, Madrid, Spain
  • Marcelino Avilés-Trigueros
    Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia and Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, Murcia, Murcia, Spain
  • Manuel A Vidal-Sanz
    Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia and Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, Murcia, Murcia, Spain
  • Jose Ramirez
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Medicina. Departamento de Inmunología, Oftalmología y ORL, Madrid, Madrid, Spain
  • Juan J. Salazar
    Instituto de Investigaciones Oftalmológicas Ramón Castroviejo. Universidad Complutense de Madrid (UCM) and OFTARED-ISCIII, Madrid, Madrid, Spain
    Facultad de Óptica y Optometría. Departamento de Inmunología, Oftalmología y ORL. UCM, Madrid, Madrid, Spain
  • Footnotes
    Commercial Relationships   Ana Ramirez, None; Jose A. Fernández-Albarral, None; Inés López Cuenca, None; Elena Salobrar-García, None; Pilar Rojas, None; Rosa de Hoz, None; Marcelino Avilés-Trigueros, None; Manuel Vidal-Sanz, None; Jose Ramirez, None; Juan J. Salazar, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2370. doi:
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      Ana Ramirez, Jose A. Fernández-Albarral, Inés López Cuenca, Elena Salobrar-García, Pilar Rojas, Rosa de Hoz, Marcelino Avilés-Trigueros, Manuel A Vidal-Sanz, Jose Ramirez, Juan J. Salazar; Microglial activation in healthy retina and experimental glaucoma during aging.. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2370.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To quantify morphological signs of microglial cell activation and P2RY12, MHC-II and CD68 expression in aged mice (15 month) compared to young adult mice (3 month) and after unilateral laser-induced ocular hypertension (OHT).

Methods : Albino Swiss mice were divided in four groups: young naïve (YN, n=6), aged naïve (AN, n=6), young OHT (YG, n=6), and aged OHT (AG, n=6). In OHT groups both eyes (OHT and contralateral) were studied. Retinal whole-mounts were immunolabeled with anti Iba-1 to quantify: i) Iba-1 + cells number (Ibacn) in outer segments (OS), outer plexiform layer (OPL) and inner plexiform layer (IPL); ii) area of retina occupied by Iba-1 + cells (Iba-1 RA) in the nerve fiber layer- ganglion cell layer (NFL-GCL): iii) cell body area of Iba-1+ cells (CbIbac) in OPL, IPL and NFL-GCL; iv) arbor area of Iba-1+ cells (AAIbac) in OPL and IPL; and v) vertical processes number of Iba-1+ cells (VPIbac) between OS and OPL. Antibodies against P2RY12 (resident microglia), CD68 (phagocytic activity) and MHC-II (microglial activation marker) were also used.

Results : In AN respect to YN the quantitative analysis showed a significant increase in the Cbibac in OPL, IPL and NFL-GCL and a significant increase in the VPibac. Both OHT eyes and contralateral eyes of YG and AG showed significant increase of Ibacn, Iba-1 RA, CbIbac and VPIbac and decrease of AAIbac, more pronounced in OHT eyes, compared to their respective naïve groups. Comparing AG vs YG showed: i) a significant decrease of Iba-1 RA and VPIbac in OHT eyes; ii) a significant increase of Ibacn (OS) and a significant decrease of CbIbac and AAIbac (IPL), in contralateral eyes. Analyzing the P2RY12, CD68 and MHC-II expression we observed: i) in YN and in YG all Iba-1+ cells were P2RY12+, except perivascular and dendritic cells, but in AG most cells were Iba-1+/P2RY12- and some cells in AN; ii) in AN, AG and YG numerous amoeboid-like CD68+ cells were found but in AG CD68+ cells with ramified appearance were also observed. In YG practically all Iba-1+ cells were MHC-II+, while in GA only some Iba-1+ cells were MHC-II+. However, in YN and AN Iba-1+ cells were MHCII-.

Conclusions : The morphological and expression differences of P2RY12, CD68 and MHC-II found in the microglial cells of AN and AG compared to those of YN and YG show that aging can increase the inflammatory process in glaucoma.

This is a 2021 ARVO Annual Meeting abstract.

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