June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Violet light suppresses lens-induced myopia via neuropsin (OPN5) in mice
Author Affiliations & Notes
  • Toshihide Kurihara
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Xiaoyan Jiang
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Machelle Pardue
    Georgia Institute of Technology, Atlanta, Georgia, United States
    Emory University, Atlanta, Georgia, United States
  • Kiwako Mori
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Shin-ichi Ikeda
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Hidemasa Torii
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Shane D’Souza
    Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States
  • Richard A Lang
    Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States
  • Kazuo Tsubota
    Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Toshihide Kurihara, Tsubota Laboratory, Inc (P), Tsubota Laboratory, Inc (F); Xiaoyan Jiang, Tsubota Laboratory, Inc (P); Machelle Pardue, None; Kiwako Mori, None; Shin-ichi Ikeda, Tsubota Laboratory, Inc (P); Hidemasa Torii, Tsubota Laboratory, Inc (P); Shane D’Souza, None; Richard Lang, BIOS (F), BIOS (P); Kazuo Tsubota, Tsubota Laboratory, Inc (I), Tsubota Laboratory, Inc (P)
  • Footnotes
    Support  KAKENHI 18K09424, EY016435, IK6 RX003134, EY027711, EY032029, EY027077
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2277. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Toshihide Kurihara, Xiaoyan Jiang, Machelle Pardue, Kiwako Mori, Shin-ichi Ikeda, Hidemasa Torii, Shane D’Souza, Richard A Lang, Kazuo Tsubota; Violet light suppresses lens-induced myopia via neuropsin (OPN5) in mice. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2277.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Myopia has become a major public health concern, particularly across much of Asia. It has been shown in multiple studies that outdoor activity has a protective effect on myopia. It is also known that short-wavelength visible violet light is the component of sunlight that appears to play an important role in preventing myopia progression in the chick and in humans. The mechanism underlying this effect has not been understood.

Methods : Lens defocus-induced myopia (LIM) in mice was performed by attaching 0 diopter (D) lenses on the left eyes and -30 D lenses on the right eyes with a frame on their skull. Combined with 50 lux of white background fluorescent lamp light from 08:00 to 20:00 every day, 400 μw/cm2 (360 nm ~ 400 nm) of violet light was added either 3 hours pre-dawn (05:00~08:00), all daytime exposure (08:00~20:00), continuous violet light, 3 hours of evening (17:00~20:00), or 3 hours of post-dusk (20:00~23:00) for three weeks of the LIM period. The violet light sensitive atypical opsin neuropsin (OPN5) is specifically deleted in the retina by crossing Chx10-Cre+ mice with Opn5flox/flox mice (OPN5 cKO).

Results : 3 hours of evening violet light combined with white light significantly suppressed both the refractive shift (p=0.006) and axial lengthening (p=0.045) compared to white light only in control mice. By contrast, OPN5 cKO mice with violet light showed a refractive shift and an axial length change indistinguishable from the normal white light condition.

Conclusions : These findings identify OPN5 expressing retinal cells as crucial for emmetropization in mice and further suggest a strategy for myopia prevention in humans.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×