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Toshihiro Kawai, Takao Nakamura, Yoshitaka Kato, Masayoshi Nakatani, Tokio Ueno; The sustained release of genipin with a Drug Delivery System inhibit an axial length elongation in the guinea pigs for 21 days. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2260.
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We evaluated an inhibitory effect of axial length (AL) elongation on form deprivation (FD) model in guinea pigs after a natural collagen crosslinker, genipin sustained release formulation (GSRF) injection.
Thirty-seven 3-week-old pigmented guinea pigs underwent monocular FD with diffusers for 21 days.Genipin solution was injected into Tenon capsule on FD eyes. Experimental design was assigned to 4 groups: Control Group ( n = 9) , Single dose Group (100 µL of 0.5% genipin solution at the 0th day, n = 10) , Multiple dose Group (100 µL of 0.5% genipin solution at days 0, 7, and 14, n = 8) , and GSRF Group (30 µL of 1.4% GSRF at the 0th day, n = 10) .AL was measured before and after FD by A-scan ultrasonography (NIDEK US-4000). Two-tailed Student’s t-test was used for statistical analysis.
The AL elongation was 0.38 ± 0.10 mm, 0.37 ± 0.12 mm, -0.02 ± 0.13 mm, and -0.03 ± 0.09 mm in the Control Group, Single dose Group, Multiple dose Group, and GSRF Group, respectively (mean ± SD).GRSF Group significantly inhibited the AL elongation compared to Control Group (p = 0.005) and Single dose Group (p = 0.012).Furthermore, there was no significant difference in AL elongation between GSRF Group and Multiple dose Group (p = 0.942).
In the FD guinea pig model, single injection of GSRF showed an inhibitory effect on AL elongation for at least 21 days.Further studies are needed to examine the safety and stability of GSRF.
This is a 2021 ARVO Annual Meeting abstract.
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