June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Elevated expression of Decorin inhibits injury-driven epithelial-mesenchymal transition in mouse lens
Author Affiliations & Notes
  • Eri Kubo
    Ophthalmology, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Shinsuke Shibata
    Ophthalmology, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Naoko Shibata
    Ophthalmology, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Satoshi Ohtsuka
    Laboratory for Experimental Animals, Kyoto Furitsu Ika Daigaku, Kyoto, Kyoto, Japan
    Medical Research Institute, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Yasuhito Yoshitomi
    Biochemistry, Kanazawa Ika Daigaku, Kahoku-gun, Japan
  • Etsuko Kiyokawa
    Oncogenic Pathology, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Yasuhito Ishigaki
    Medical Research Institute, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Hideto Yonekura
    Biochemistry, Kanazawa Ika Daigaku, Kahoku-gun, Japan
  • Hiroshi Sasaki
    Ophthalmology, Kanazawa Ika Daigaku, Kahoku-gun, Ishikawa, Japan
  • Dhirendra P Singh
    Ophthalmology and Visual Science, University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Footnotes
    Commercial Relationships   Eri Kubo, Novartis Pharma (F), NPO "Eyes Arc" (S); Shinsuke Shibata, None; Naoko Shibata, None; Satoshi Ohtsuka, None; Yasuhito Yoshitomi, None; Etsuko Kiyokawa, None; Yasuhito Ishigaki, None; Hideto Yonekura, None; Hiroshi Sasaki, Alcon Japan (F), Alcon Pharma (F), AMO Japan (F), Heart Optical (R), HOYA, Izone Japan (F), Johnson & Johnson (F), Kowa Pharmaceutical (F), Neitz (F), NPO "Eyes Arc" (S), Otsuka Pharmaceutical (F), Santen Pharmaceutical (F), Senju Pharmaceutical (F), Tomiki Medical Instruments (F); Dhirendra Singh, None
  • Footnotes
    Support  JSPS Grants-in-Aid for Scientific Research C 23592588 (to SS); the National Eye Institute, National Institute of Health [grant number EY024589 (to D.P.S.); Research for Preventing Blindness (to D.P.S.)
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2059. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Eri Kubo, Shinsuke Shibata, Naoko Shibata, Satoshi Ohtsuka, Yasuhito Yoshitomi, Etsuko Kiyokawa, Yasuhito Ishigaki, Hideto Yonekura, Hiroshi Sasaki, Dhirendra P Singh; Elevated expression of Decorin inhibits injury-driven epithelial-mesenchymal transition in mouse lens. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2059.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Decorin (Dcn) is a dermatan sulfate proteoglycan which belongs to the small leucine-rich proteoglycan family and plays critical roles in controlling extracellular matrix assembly homeostasis. This is highly upregulated in mouse and rat posterior capsular opacification (PCO) tissues after extracapsular lens extraction surgery. Herein, we aim to explore the biological role of decorin in the development or prevention of PCO in mice lenses overexpressing human DCN (hDCN).

Methods : All animal and recombinant DNA experiments were approved by the Kanazawa Medical University Ethics Committee. We generated lens-specific hDCN-transgenic mice (hDCN-Tg) using a plasmid in which Pax6-human alpha crystallin (P6a) composite promoter drives hDCN cDNA. Histological analysis of postnatal day (PD) 2, 8 and 48 weeks-old hDCN-Tg and wild type (WT) mice were performed by using Hematoxylin & Eosin (H&E) staining. Expression of DCN in hDCN-Tg on PD2 was confirmed by histochemical staining for lacZ with 5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) and immunostaining using anti-Dcn Ab. The lens injury model as mouse PCO model was generated in 14 weeks-old hDCN-Tg and WT mice. The histological patterns were evaluated by H&E staining and immunohistochemical analysis using anti-α smooth muscle actin (αSMA) antibody.

Results : X-gal was stained in cytoplasm of lens epithelial cells (LECs) at equatorial to bow region and primary lens fiber from PD2 hDCN-Tg mice. hDCN protein was expressed in LECs and surface fibers in lens sections from PD2-hDCN-Tg. However, X-gal staining and DCN expression was not observed in lens from PD2-WT mice. In histological observation of eye lens in PD2, adult 8 and 48 weeks-old hDCN-Tg, morphological changes were not observed compared to wild type as control. Importantly, overexpression of DCN inhibited fibroblastic EMT changes and αSMA expression observed in mouse wound healing of lens surface in hDCN-Tg.

Conclusions : Our findings reveal that enhanced expression of DCN play a role in preventing EMT of LECs and has potential beneficial effect in the intervention of PCO progression.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×