June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Biomechanical characterization of a Col5a1+/- mouse model of classic type Ehlers-Danlos syndrome with tensile extensometry and OCT elastography
Author Affiliations & Notes
  • Sabine Kling
    D-ITET, ETH Zurich, Switzerland
  • Emilio Torres-Netto
    UniversitatsSpital Zurich, Zurich, Switzerland
    CABMM, Universitat Zurich, Zurich, ZH, Switzerland
  • Hormoz Abdshahzadeh
    CABMM, Universitat Zurich, Zurich, ZH, Switzerland
  • Edgar Espana
    Morsani College of Medicine, University of South Florida, Tampa, Florida, United States
  • Farhad hafezi
    CABMM, Universitat Zurich, Zurich, ZH, Switzerland
    ELZA Institute AG, Dietikon, Zurich, Switzerland
  • Footnotes
    Commercial Relationships   Sabine Kling, None; Emilio Torres-Netto, None; Hormoz Abdshahzadeh, None; Edgar Espana, None; Farhad hafezi, None
  • Footnotes
    Support  SNF Ambizione PZ00P2_174113
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2040. doi:
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      Sabine Kling, Emilio Torres-Netto, Hormoz Abdshahzadeh, Edgar Espana, Farhad hafezi; Biomechanical characterization of a Col5a1+/- mouse model of classic type Ehlers-Danlos syndrome with tensile extensometry and OCT elastography. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2040.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To quantify biomechanical properties in a Col5a1+/- mouse model for classic type Ehlers-Danlos syndrome (EDS) and to compare two different measurement approaches suited for murine corneal mechanical characterization.

Methods : A total of 14 adult eyes, 90 days old, of a Col5a1-haploinsufficient mouse model (Col5a1 het) and 14 eyes of the same age of wild-type littermates (wt) were analyzed by optical coherence elastography (OCE) and 2D stress-strain extensometry. Quasi-static OCE was conducted non-invasively during ambient pressure modulation by -3 mmHg. Corneal displacements were analyzed by phase-difference processing. 2D extensometry measurements consisted of a pre-conditioning cycle, followed by a stress-relaxation test and finally a rupture test.

Results : Compared to wt corneas, Col5a1 het corneas had a thinner corneal thickness (125±11 vs 148±10 mm, p<0.001). Short-term elastic modulus in Col5a1 het corneas was significantly increased in OCT measurements (506±88 vs 430±103 kPa, p=0.023), and the same trend was observed in stress-strain extensometry (30.7±12.1 vs 21.5±5.7 kPa, p=0.057). In contrast, in stress relaxation tests, Col5a1 het corneas experienced a stronger relaxation (55% vs 50%, p=0.010). The two distinct behaviors indicate increased short-term stiffness and reduced long-term stiffness in het corneas.

Conclusions : A reduced expression of collagen V in cornea seems to predominantly affect the viscoelastic properties of the tissue. The results presented here support and rationalize the counterintuitive clinical reported findings, in which even thinner corneas with potential alterations in the structure of collagen manage to maintain a normal topographic pattern.

This is a 2021 ARVO Annual Meeting abstract.


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