June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Bioprinting of a spherical hydrogel dome containing uveal melanoma cells to test the functionality of custom brachytherapy implants
Author Affiliations & Notes
  • Philippe Gros-Louis
    Department of Ophthalmology, Universite Laval Faculte de medecine, Quebec City, Quebec, Canada
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
  • Sophie Lemay
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
    Department of Mining, Metallurgy and Materials Engineering, Universite Laval, Quebec City, Quebec, Canada
  • Théophraste Lescot
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
    Department of Mining, Metallurgy and Materials Engineering, Universite Laval, Quebec City, Quebec, Canada
  • Léo Piquet
    Department of Ophthalmology, Universite Laval Faculte de medecine, Quebec City, Quebec, Canada
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
  • Claudine Bellerive
    Department of Ophthalmology, Universite Laval Faculte de medecine, Quebec City, Quebec, Canada
  • Marc-André Fortin
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
    Department of Mining, Metallurgy and Materials Engineering, Universite Laval, Quebec City, Quebec, Canada
  • Solange Landreville
    Department of Ophthalmology, Universite Laval Faculte de medecine, Quebec City, Quebec, Canada
    Division of Regenerative Medicine, CHU de Quebec-Universite Laval, Quebec City, Quebec, Canada
  • Footnotes
    Commercial Relationships   Philippe Gros-Louis, None; Sophie Lemay, None; Théophraste Lescot, None; Léo Piquet, None; Claudine Bellerive, None; Marc-André Fortin, None; Solange Landreville, None
  • Footnotes
    Support  IRSC, Fondation du CHU de Québec, Fondation de l'Université Laval
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2869. doi:
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      Philippe Gros-Louis, Sophie Lemay, Théophraste Lescot, Léo Piquet, Claudine Bellerive, Marc-André Fortin, Solange Landreville; Bioprinting of a spherical hydrogel dome containing uveal melanoma cells to test the functionality of custom brachytherapy implants. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2869.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The radiation received by patients with uveal melanoma (UM) treated with brachytherapy is often poorly distributed for cases of non-spherical tumors. The manufacture of personalized brachytherapy implants (PBI) would improve patient comfort during treatment and reduce post-irradiation ocular complications. Our objective is to develop an in vitro experimental model reproducing the anatomy of the eye with a choroidal tumor in order to test the efficacy and functionality of PBI.

Methods : Our printed model will include the main elements of the extracellular matrix of the choroid and sclera, as well as choroidal fibroblasts (CF), choroidal melanocytes (CM) and UM cells (92.1, Mµ2 and Mel270). Cell death ratio (CDR) was quantified by fluorescence (LIVE/DEADTM Viability/Cytotoxicity Kit) to observe the response of choroidal and UM cell lines when embedded in a hydrogel for 0, 4 or 7 days (T0, T4 and T7). Surgical evaluation of PBI (N = 5) on freshly enucleated human eyes was done and scored (out of 35) based on mechanical properties and surgical precision (MPSP) for each prototype in order to compare them between each other and to standard brachytherapy implants (SBI).

Results : The cell line with the highest CDR was CF (T0= 23.86±2.53%) and the UM cell line with the lower CDR was Mel270 (T7= 0.79±0.42%). The CDR of the three other cell lines varied between 13.75±1.56% and 0.83±0.28%. Compared to the SBI MPSP score of 21, the two prototypes with flexible eyelets scored higher with a combined MPSP score of 27 and the resin prototype scored the highest of all prototypes with a MPSP score of 29. Both prototypes (with flexible eyelets or in resin) had higher score mainly in mechanical properties, while both the prototype with rigid eyelets and the prototype with full outline had lower scores in surgical precision, with the same MPSP score of 19.

Conclusions : Our results showed a low CDR with all 5 cell lines embedded in our model of hydrogel and identified two potential prototypes of PBI. However, bioprinting the dome and its irradiation will make it possible to better characterize the cellular structural changes post-irradiation, and to further optimize the prototypes of PBI. This would allow the development of an in vitro 3D model of choroidal melanoma for the validation of new forms of implants personalized according to the geometry of the tumor.

This is a 2021 ARVO Annual Meeting abstract.

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