June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Myo/Nog Cells Migrate to Areas of Stress Within the Eye in a Mouse Model of Glaucoma
Author Affiliations & Notes
  • Karanveer Johal
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Paul Lecker
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • John Spikes
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jake Bernstein
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Alexa McGrath
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Victoria MacPherson
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Rushil Brahmbhatt
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Mark Martin
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Brent Bisco
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Sarah Adams
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Muhannah Hossain
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jashanveer Johal
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Grzegorz Gorski
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Arturo Bravo Nuevo
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Jacquelyn V Gerhart
    Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Karanveer Johal, None; Paul Lecker, None; John Spikes, None; Jake Bernstein, None; Alexa McGrath, None; Victoria MacPherson, None; Rushil Brahmbhatt, None; Mark Martin, None; Brent Bisco, None; Sarah Adams, None; Muhannah Hossain, None; Jashanveer Johal, None; Grzegorz Gorski, None; Arturo Bravo Nuevo, None; Jacquelyn Gerhart, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2752. doi:
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      Karanveer Johal, Paul Lecker, John Spikes, Jake Bernstein, Alexa McGrath, Victoria MacPherson, Rushil Brahmbhatt, Mark Martin, Brent Bisco, Sarah Adams, Muhannah Hossain, Jashanveer Johal, Grzegorz Gorski, Arturo Bravo Nuevo, Jacquelyn V Gerhart; Myo/Nog Cells Migrate to Areas of Stress Within the Eye in a Mouse Model of Glaucoma. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2752.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Myo/Nog cells express MyoD, Bai1 and the bone morphogenic protein inhibitor Noggin. They are critical for normal embryonic development, including eye morphogenesis. In adults, they respond to stress and injury. Depletion of Myo/Nog cells in the mouse retina was associated with increased photoreceptor cell death, while their addition to the vitreous preserved function and reduced cell death following light damage in the rat. Here we examine the behavior of endogenous and exogenous Myo/Nog cells in response to increased intraocular pressure (IOP) in a mouse model of glaucoma.

Methods : Glaucoma was induced in the right eye of each animal by impeding the outflow of the aqueous humor with microbeads injected into the anterior cavity of C57BL/6J mice (D0). IOP was measured periodically from D0 to D32. Seven days after insertion of microbeads, three of four groups received bilateral AC injections of PBS, unsorted brain cells or Myo/Nog cells isolated from the brain with the G8 monoclonal antibody and prelabeled with a fluorescent dye. The fourth group received no follow up injections. Eyes were collected at D32, and immunofluorescence localization of exogenous and endogenous G8+ cells was performed.

Results : Myo/Nog cells injected into the anterior chamber were tracked to areas of stress, such as the trabecular meshwork, the ciliary body and the canal of Schlemm. The highest concentration of Myo/Nog cells were found in the cornea and retinal ganglion cell layer. H&E staining showed a statistical deficit in living retinal ganglion cells within the RGC layer of the glaucomous eye compared to the non-glaucomous eye among the PBS and mixed population treatment groups, and no statistical loss of cells within the glaucomous eye compared to the non-glaucomous in the exogenous Myo/Nog treatment group.

Conclusions : Myo/Nog cells increase in number in response to elevated IOP and are concentrated in areas of stress in the eye. Endogenous Myo/Nog cells were increased around the optic nerve and periphery of the retina compared to normal retinas. Myo/Nog cells injected into the anterior chamber showed evidence of integration into tissues that are affected by impaired aqueous humor outflow and migrate to areas of increased endogenous Myo/Nog concentration within the eye. Injection of exogenous Myo/Nog cells also showed preservation of retinal ganglion cells in glaucomous eyes.

This is a 2021 ARVO Annual Meeting abstract.

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