June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Platelets enhance the antifungal activity of neutrophils
Author Affiliations & Notes
  • Wei Si
    Zhengzhou University People's Hospital,Henan Provincial People's Hospital,Henan Eye Institute, China
  • Fenfen Zhang
    People's Hospital of Dezhou, China
  • Hongmin Zhang
    Zhengzhou University People's Hospital,Henan Provincial People's Hospital,Henan Eye Institute, China
  • Susu Liu
    Zhengzhou University People's Hospital,Henan Provincial People's Hospital,Henan Eye Institute, China
  • Footnotes
    Commercial Relationships   Wei Si, None; Fenfen Zhang, None; Hongmin Zhang, None; Susu Liu, None
  • Footnotes
    Support  National Natural Science Foundation of China(No:81770902)
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2683. doi:
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      Wei Si, Fenfen Zhang, Hongmin Zhang, Susu Liu; Platelets enhance the antifungal activity of neutrophils. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2683.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : In the previous experiment, platelet, neutrophil aggregation were observed in the focus area of fungal keratitis in mice, but the relationship between platelet and neutrophil was not clear. Therefore, the effects and mechanisms of the antifungal activity of neutrophils enhanced by platelets were studied.

Methods : All fungi used for experiments was the standard strain of Fusarium solani. Neutrophils and platelets were drawn from human peripheral venous and purified from whole blood respectively. Three groups were divided in this experiments: spore (S) group, spore + neutrophil (S+N) group co-incubated spores and isolated neutrophils together and spore + neutrophil + platelet (S+N+PL) group co-incubated spores, isolated neutrophils and platelets. The growth of fungi or neutrophils or platelets was observed and the pictures were took at specified time points used by a spinning disk confocal microscope. The rate of phagocytosis, spore germination, hyphae length and apoptosis in each group were calculated.

Results : The rate of phagocytosis of neutrophils in S+N+PL group were higher at 1h, 2h and 4h compared with that in S+N group (P < 0.05). The rate of spore germination was lower in S+N+PL group compared with that in S+N group (P < 0.05). In addition, with longer incubated time, the apoptosis of neutrophils grown higher. Until 18h, the apoptosis rate of N group was grown as high as 74.56%. The highest apoptosis rate of neutrophils was in N group, followed by S+N group and S+N+PL group at each time point (P = 0.000). The apoptosis rate in S+N group was significantly higher than that in S+N+PL group at 6h, 10h, 14h and 18h (P < 0.01).

Conclusions : Platelets could enhance neutrophils to engulf fungal spores,restrict fungal germination,delay the hyphae growth and reduce apoptosis of neutrophil. These results indicated that the platelets play an important role in anti-fungal field.

This is a 2021 ARVO Annual Meeting abstract.

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