June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Voluntary exercise preserves visual function and prevents increased inflammatory cytokine expression in an adult mouse model of autosomal dominant retinitis pigmentosa.
Author Affiliations & Notes
  • Alicia Chacko
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
  • Austin Karesh
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
  • Katie Bales
    Atlanta VA Center for Visual & Neurocognitive Rehabilitation, Decatur, Georgia, United States
    Department of Ophthalmology, Emory University, Atlanta, Georgia, United States
  • Kelleigh Hogan
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
  • Alyssa Pybus
    Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States
  • Levi Wood
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
    Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States
  • Jeffrey H Boatright
    Atlanta VA Center for Visual & Neurocognitive Rehabilitation, Decatur, Georgia, United States
    Department of Ophthalmology, Emory University, Atlanta, Georgia, United States
  • Machelle T Pardue
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States
    Atlanta VA Center for Visual & Neurocognitive Rehabilitation, Decatur, Georgia, United States
  • Footnotes
    Commercial Relationships   Alicia Chacko, None; Austin Karesh, None; Katie Bales, None; Kelleigh Hogan, None; Alyssa Pybus, None; Levi Wood, None; Jeffrey Boatright, None; Machelle Pardue, None
  • Footnotes
    Support  NIH Core Grant P30 EY06360, NIH R01 EY028859, NIH Health Cell and Tissue Engineering Biotechnology Training Grant T32-GM008433, and Dept of Veterans Affairs Research Career Scientist Award RX003134, VA RR&D C9246C, VA RR&D C1924P I21RX001924, NIH P30EY06360, Abraham and Phyllis Katz Foundation, Research to Prevent Blindness, Foundation for Atlanta Veterans Education and Research
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 2596. doi:
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      Alicia Chacko, Austin Karesh, Katie Bales, Kelleigh Hogan, Alyssa Pybus, Levi Wood, Jeffrey H Boatright, Machelle T Pardue; Voluntary exercise preserves visual function and prevents increased inflammatory cytokine expression in an adult mouse model of autosomal dominant retinitis pigmentosa.. Invest. Ophthalmol. Vis. Sci. 2021;62(8):2596.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Previous studies support that exercise could potentially be used as a neuroprotective method in patients with retinitis pigmentosa (RP) as well as in animal models of RP. The underlying molecular mechanisms of exercise-induced neuroprotection are elusive. This study investigates the neuroprotective effects of voluntary exercise on retinal structure, function and inflammatory response in an adult mouse model of autosomal dominant retinitis pigmentosa (adRP).

Methods : Adult I307N rhodopsin mice (n=25) were divided into active or inactive groups and received free-spinning or locked running wheels, respectively. After exercising for two weeks, the mice received either a 5-minute period of bright light (“BL”; 6000 lux) or natural light (“dim”; 50 lux). At one and two weeks post-BL, optomotor response (OMR) and electroretinograms (ERG) were measured. After four weeks of exercise, the mice were euthanized and eyes were enucleated for histological and multiplexed cytokine protein expression analysis.

Results : OMR revealed significantly greater spatial frequency and contrast sensitivity thresholds for the active+BL group compared to inactive+BL (p<0.001). Histological analysis showed a preservation of the outer nuclear layer between active+BL and inactive+BL (p=0.007). ERG a- and b-wave amplitudes in active+BL trended higher compared to inactive+BL, however they did not reach statistical significance (p>0.05). Cytokine expression profiles were different for active+BL and inactive+BL animals with trending reductions in interferon gamma-induced protein 10 (IP-10; p=0.085) and keratinocyte-derived chemokine (KC; p=0.093) in active+BL animals, which are involved in chemotactic signaling of microglia and leukocytes.

Conclusions : Our results show that voluntary exercise is protective against retinal degeneration in an adult mouse model of adRP. Exercise preserved visual function and prevented increased expression of inflammatory cytokine expression. These results suggest that exercise regimens should be assessed as a therapeutic intervention to delay progression of RP in patients.

This is a 2021 ARVO Annual Meeting abstract.

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