June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Optical coherence tomography imaging-based evaluation of lesion characteristics in a rabbit model of proliferative vitreoretinopathy
Author Affiliations & Notes
  • Yuchen Lu
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Ying Liu
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Minda McNally
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • William Schubert
    Bayer AG, Leverkusen, Wuppertal, Germany
  • Melanie Adams
    Research Animal Resources, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Jiang Qian
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Seth Blackshaw
    Solomon H. Synder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Charles Eberhart
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Khaled Nassar
    Bayer AG, Leverkusen, Wuppertal, Germany
  • Mandeep S Singh
    Johns Hopkins Medicine Wilmer Eye Institute, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Yuchen Lu, None; Ying Liu, None; Minda McNally, None; William Schubert, Bayer AG (E); Melanie Adams, None; Jiang Qian, Bayer AG (F); Seth Blackshaw, Bayer AG (F); Charles Eberhart, Bayer AG (F); Khaled Nassar, Bayer AG (E); Mandeep Singh, Bayer AG (F), Bayer AG (C)
  • Footnotes
    Support  The work is part of a research collaboration with Bayer AG
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 3625. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yuchen Lu, Ying Liu, Minda McNally, William Schubert, Melanie Adams, Jiang Qian, Seth Blackshaw, Charles Eberhart, Khaled Nassar, Mandeep S Singh; Optical coherence tomography imaging-based evaluation of lesion characteristics in a rabbit model of proliferative vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2021;62(8):3625.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Proliferative vitreoretinopathy (PVR) is an important cause of irreversible vision loss for which no treatment other than surgery is available. Large animal models of induced PVR have been used to study disease mechanisms and to test novel pharmacologic treatments. Photography-based staging scales in these models classify lesion severity by macroscopic features but do not provide data regarding microscopic retinal structure. Here we aimed to study the use of optical coherence tomography (OCT) imaging in characterizing the microscopic in vivo features of retinal lesions in a rabbit model of induced PVR.

Methods : All animal procedures were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the Johns Hopkins Animal Care and Use Committee. PVR was induced unilaterally in specific pathogen free Dutch Belted rabbits by vitrectomy, retinotomy, retinal detachment, platelet-rich plasma injection, and cryotherapy. At baseline and different time points after PVR induction, in vivo lesion severity was documented by color fundus photography and staged according to the Fastenberg scale. OCT imaging (EnFocus OCT, Leica Microsystems) was performed at each time point. Qualitative and quantitative features were extracted from OCT images by a masked observer.

Results : The retinas (N=9) of lesioned rabbits were imaged at baseline and at time points ranging from 4 hours to 30 days after PVR induction. PVR lesion severity ranged from Fastenberg stages 3 to 5. Qualitative OCT features that were analyzed included retinal thickening, epiretinal membrane, vitreoretinal traction, media opacity, and retinal detachment. OCT features that could be quantified included retinal thickness and retinal detachment height. Cataract formation impeded OCT imaging in several eyes.

Conclusions : OCT imaging may provide detailed information regarding in vivo retinal structure in rabbit eyes with PVR across the severity spectrum. Qualitative OCT lesion descriptors could reveal abnormalities in the epiretinal, retinal, and subretinal locations. The development of objective and quantifiable OCT-based biomarkers in animal models of PVR might facilitate further preclinical research on developing novel treatment modalities.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×