June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Objective measurement of anterior chamber cells in children with uveitis using anterior segment optical coherence tomography
Author Affiliations & Notes
  • Edmund Tsui
    Ophthalmology, UCLA Stein Eye Institute, Los Angeles, California, United States
    University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Judy L. Chen
    Ophthalmology, UCLA Stein Eye Institute, Los Angeles, California, United States
  • Nicholas J Jackson
    Division of General Internal Medicine and Health Services Research, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Omar Leyva
    Ophthalmology, UCLA Stein Eye Institute, Los Angeles, California, United States
  • Haroon A Rasheed
    University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Elmira Baghdasaryan
    Doheny Eye Institute, Los Angeles, California, United States
  • Simon Fung
    Ophthalmology, UCLA Stein Eye Institute, Los Angeles, California, United States
    University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Deborah K McCurdy
    Pediatrics, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Srinivas R Sadda
    Doheny Eye Institute, Los Angeles, California, United States
    University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Gary N Holland
    Ophthalmology, UCLA Stein Eye Institute, Los Angeles, California, United States
    University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, United States
  • Footnotes
    Commercial Relationships   Edmund Tsui, Kowa Company Ltd (F); Judy Chen, None; Nicholas Jackson, None; Omar Leyva, None; Haroon Rasheed, None; Elmira Baghdasaryan, None; Simon Fung, None; Deborah McCurdy, None; Srinivas Sadda, 4DMT (C), Allergan (C), Amgen (C), Carl Zeiss Meditec (F), Carl Zeiss Meditec (S), Centervue (C), Centervue (S), Heidelberg Engineering (C), Heidelberg Engineering (S), Nidek (S), Novartis (C), Optos (C), Optos (F), Optos (S), Oxurion (C), Regeneron (C), Topcon (S); Gary Holland, None
  • Footnotes
    Support  Knights Templar Eye Foundation, Thrasher Research Fund, Research to Prevent Blindness, Inc
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 3477. doi:
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    • Get Citation

      Edmund Tsui, Judy L. Chen, Nicholas J Jackson, Omar Leyva, Haroon A Rasheed, Elmira Baghdasaryan, Simon Fung, Deborah K McCurdy, Srinivas R Sadda, Gary N Holland; Objective measurement of anterior chamber cells in children with uveitis using anterior segment optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2021;62(8):3477.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the feasibility of anterior segment optical coherence tomography (AS-OCT) for the measurement of anterior chamber (AC) cells in children with uveitis and to evaluate different AS-OCT acquisition modes for measuring AC cells.

Methods : We prospectively enrolled children younger than 18 years of age with histories of uveitis involving the anterior chamber and healthy children without histories of uveitis or intraocular surgery as controls. All children underwent a comprehensive eye examination and grading of AC cell using the Standardization of Uveitis Nomenclature (SUN) grading system. All were imaged using the Optovue Avanti RTVue XR AS-OCT. Each underwent AS-OCT scans using two acquisition modes: a single cross-sectional line scan and 8 cross-sectional line scans in an asterisk formation. Two independent, masked graders manually counted cells (represented by hyperreflective foci in AS-OCT images). Rater agreement was assessed using intraclass correlation (ICC).

Results : We evaluated 59 eyes of 30 children with histories of uveitis (mean age 12.1 years, range 3-17 years; 19 [63%] females) and 40 eyes of 20 control children (mean age 10.9 years, range 4-17 years; 9 [45%] females). Of 59 eyes with uveitis, 32 (54%) had Grade 0 cell; 12 (20.3%) had 0.5+ cell; 5 (8.5%) had 1+ cell; 8 (13.6%) had 2+ cell; and 2 (3.4%) had 3+ cell on clinical examination. For line scans, mean OCT cell counts for each grade were: Grade 0, 0.17 (range 0-5); 0.5+, 0.88 (0-3); 1+, 2.9 (0-7); 2+, 9.7 (0-25); and 3+, 25.8 (17-35). For asterisk formation scans, mean OCT cell counts for each grade were: Grade 0, 0.11 (range, 0-4); 0.5+, 0.49 (0-3); 1+, 1.4 (0-6); 2+, 5.5 (0-18); and 3+, 22.0 (12-31). ICC of graders for line and asterisk formation scans were 0.87 and 0.90, respectively, with no significant difference between acquisition modes (95% confidence interval -0.04 to 0.14). No eyes of control children had cells on AS-OCT images.

Conclusions : Quantification of AC cell in children with uveitis is feasible with AS-OCT and has excellent reliability between different graders. Given the similarity in cell counts between line and asterisk formation scans, AS-OCT line scans may be a more suitable option given the relative speed of image acquisition compared to asterisk formation scans, especially in children.

This is a 2021 ARVO Annual Meeting abstract.

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