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Chang Ho Yoon, JinSuk Ryu, Mee Kum Kim; Aging-dependent gut microbiome dysbiosis is associated with dry eye severity in C57BL/6 male mouse model. Invest. Ophthalmol. Vis. Sci. 2021;62(8):3449.
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© ARVO (1962-2015); The Authors (2016-present)
Emerging evidence have demonstrated that gut dysbiosis contributes to the pathophysiology or exacerbation of ocular diseases including dry eye disease. However, the relationship between aging-related changes in gut microbiota and dry eye disease have not been elucidated. We investigated the association between aging-dependent microbiome changes and dry eye severity in C57BL/6 male mice.
Eight-week-old (8W, n=15), one-year-old (1Y, n=10), and two-year-old (2Y, n=8) C57BL/6 male mice were used. Dry eye severity was assessed by corneal staining scores and tear secretion. Bacterial genomic 16s rRNA from feces was analyzed. Main outcomes were microbiome compositional differences among the groups and their correlation to dry eye severity. The Kruskal–Wallis test followed by Dunn’s post hoc test was used for comparison between the three groups. Univariate analysis was used to identify the microbiome composition associated with the dry eye severity. Multivariate analysis was used to eliminate confounding age factors.
In aged mice (1Y and 2Y), corneal staining increased and tear secretion decreased with statistical significance. Gut microbiome α-diversity was not different among the groups. However, β-diversity was significantly different among the groups. In univariate analysis, phylum Firmicutes, Proteobacteria, and Cyanobacteria, Firmicutes/Bacteroidetes ratio, and genus Alistipes, Bacteroides, Prevotella, Paraprevotella, and Helicobacter were significantly related to dry eye severity. After adjustment of age, multivariate analysis revealed phylum Proteobacteria, Firmicutes/Bacteroidetes ratio, and genus Lactobacillus, Alistipes, Prevotella, Paraprevotella, and Helicobacter to be significantly associated with dry eye severity.
Our study suggests that aging-dependent changes in microbiome composition are related to severity of dry eye signs in C57BL/6 male mice.
This is a 2021 ARVO Annual Meeting abstract.
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