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Derek Orshan, Andrew Tirsi, Vasiliki Gliagias, Benny Wong, Joby Tsai, Peter H Derr, Sung Chul Park, Stephen A Obstbaum, Celso Tello; Correlations Between Pattern Electroretinography and Estimated Retinal Ganglion Cell Count in Glaucoma Suspects. Invest. Ophthalmol. Vis. Sci. 2021;62(8):3386.
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© ARVO (1962-2015); The Authors (2016-present)
Combined structure-function index (CSFI) which derives retinal ganglion cells (RGC) estimates by combining standard automated perimetry (SAP) and optical coherence tomography (OCT) measurements into an age-adjusted algorithm, has been proven to significantly correlate with histologically derived count. In this prospective cross-sectional study, we investigated the relationship between estimated RGC count and steady state pattern electroretinogram (ssPERG) parameters in glaucoma suspects (GS).
Ten subjects (60% female, 20 eyes) were enrolled in this study conducted at the Manhattan Eyes, Ears, and Throat Hospital. Subjects were divided into two groups of GS with normal RGC function and GS with RGC dysfunction based upon ssPERG testing results. All study subjects underwent comprehensive ophthalmologic examinations including OCT, SAP and ssPERG. RGC counts were estimated using the CSFI, a statistical model that combines structural [OCT-derived average retinal nerve fiber layer thickness (aRNFLT)] and functional [SAP-derived mean deviation 24-2 (MD)] parameters. The following ssPERG parameters Magnitude (Mag), MagnitudeD (MagD) and MagnitudeD/Magnitude ratio (MagD/Mag ratio) were used in the analysis.
Independent sample t-tests revealed that all ssPERG parameters, estimated RGC count, aRNFLT, and MD were significantly reduced in GS with RGC dysfunction relative to control subjects (p< 0.015 for all parameters). A significant reduction in estimated RGC count was found in the GS with RGC dysfunction (752103 ±97827 vs 1023711±99364) respectively (p< 0.001). Pearson and partial (age-adjusted) correlation analyses revealed that all ssPERG parameters significantly correlated with MD (r>0.46, p<0.049. MagD and MagD/Mag ratio significantly correlated with aRNFLT (r >0.47, p < 0.044), with average ganglion cell layer and inner plexiform layer (GCL/IPL) thickness (r > 0.47, p<0.042). Pearson and partial (age-adjusted) correlation analyses revealed stronger linear relationships between all ssPERG parameters and estimated RGC count (r>0.58, p≤0.009) than between ssPERG and independent measures of CSFI.
Glaucoma suspects with RGC dysfunction present with decreased estimated RGC count when compared to controls, and ssPERG parameters correlate stronger with the estimated RGC count than with independent measures of CSFI.
This is a 2021 ARVO Annual Meeting abstract.
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