June 2021
Volume 62, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2021
Effect of lutein in olive oil in the mouse oxygen-induced retinopathy model
Author Affiliations & Notes
  • Kwan Wun Tsang
    Department of Ophthalmology, The University of Hong Kong, Hong Kong, Hong Kong
  • Antonia M Joussen
    Ophalmology, Charité – University Medicine Berlin, Berlin, Germany
  • Amy CY Lo
    Department of Ophthalmology, The University of Hong Kong, Hong Kong, Hong Kong
  • Footnotes
    Commercial Relationships   Kwan Wun Tsang, None; Antonia Joussen, None; Amy Lo, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2021, Vol.62, 3018. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kwan Wun Tsang, Antonia M Joussen, Amy CY Lo; Effect of lutein in olive oil in the mouse oxygen-induced retinopathy model. Invest. Ophthalmol. Vis. Sci. 2021;62(8):3018.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Retinopathy of prematurity (ROP), a proliferative retinopathy in preterm babies, is an oxidative stress associated disease. Lutein as anti-oxidative agent is a potential therapeutic agent for ROP. To improve the bioavailability of lutein, olive oil was used to dissolve lutein. To investigating the effect of lutein in olive oil in ROP progression, retinal vasculature and function were examined in mouse oxygen-induced retinopathy (OIR) model.

Methods : C57Bl/6J mother and their pups were placed into 75% oxygen chamber from postnatal day (P) 7 to P12. DMSO, olive oil alone, lutein in DMSO or lutein in olive oil (0.2mg/kg) were intraperitoneally injected into the pups starting from P12 daily. Retinal vasculature was examined in isolectin-stained retinal flat mounts at P14 and P17. Retinal function was assessed at P17 using scotopic electroretinography (ERG).

Results : Olive oil alone, lutein in DMSO and lutein in olive oil did not affect the body weight under normal room air (RA) condition or after OIR at P14 and 17. Under normal RA, the pups receiving lutein in DMSO have higher a-wave and b-wave amplitudes at P17 without affecting the normal retinal vascular development when compared with DMSO, olive oil alone or lutein in olive oil treated group. After OIR, olive oil or lutein in olive oil treated groups have a larger avascular area (DMSO: 26.4±2.9%; olive oil: 32.3±2.6%; lutein in DMSO: 26.2±2.7%; lutein in olive oil: 32.4±3.4%). At P17, although no significant difference was observed in the size of neovascularization, olive oil alone, lutein in DMSO and lutein in olive oil treated pups have a larger avascular area (DMSO: 13.5± 2.8%; olive oil: 17.3±7.2%; lutein in DMSO: 18.1±1.6%; lutein in olive oil: 16.6±5.0%). Most importantly, lutein in olive oil treated pups have higher a-wave and b-wave amplitudes with shorter implicit times.

Conclusions : Lutein in olive oil treated pups displayed an improvement of retinal function including the function of photoreceptors, bipolar cells and Müller cells after OIR, suggesting that lutein in olive oil may preserve retinal function after OIR despite limited effects in vascular protection.

This is a 2021 ARVO Annual Meeting abstract.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×