August 2021
Volume 62, Issue 11
Open Access
ARVO Imaging in the Eye Conference Abstract  |   August 2021
Application of laser Doppler holography in the anterior segment to image blood flow and assess the corneal and lens transparency
Author Affiliations & Notes
  • Leo Puyo
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
    Institute of Biomedical Optics, University of Lübeck, Lübeck, Germany
  • Clementine David
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Rana Saad
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Sami Saad
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Josselin Gautier
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Vincent Borderie
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Jose-Alain Sahel
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
    Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States
  • Michel Paques
    Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Michael Atlan
    Institut Langevin, Paris, Île-de-France, France
  • Footnotes
    Commercial Relationships   Leo Puyo, None; Clementine David, None; Rana Saad, None; Sami Saad, None; Josselin Gautier, None; Vincent Borderie, None; Jose-Alain Sahel, None; Michel Paques, None; Michael Atlan, None
  • Footnotes
    Support  European Research Council (Synergy HELMHOLTZ #610110). Agence Nationale de la Recherche (FOReSIGHT ANR-18-IAHU-0001). Region Ile-de-France (EX047007 - SESAME 2019 - 4DEye).
Investigative Ophthalmology & Visual Science August 2021, Vol.62, 7. doi:
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      Leo Puyo, Clementine David, Rana Saad, Sami Saad, Josselin Gautier, Vincent Borderie, Jose-Alain Sahel, Michel Paques, Michael Atlan; Application of laser Doppler holography in the anterior segment to image blood flow and assess the corneal and lens transparency. Invest. Ophthalmol. Vis. Sci. 2021;62(11):7.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Laser Doppler holography (LDH) is a full-field imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We report here on imaging the anterior segment of the eye with LDH to explore its potential clinical uses.

Methods : We have used the very same instrument as the one previously used for retinal and choroidal blood flow imaging, with the same digital processing based on a short-time Fourier transform analysis of the Doppler induced fluctuations. The numerical reconstruction of the holograms was adapted to have the anterior segment in focus instead of the retina. The instrument was used in healthy eyes and in cases of cataract and corneal neovascularization.

Results : We were able to image blood flow in the conjunctiva, episclera, iris, as well as in corneal neovessels. We also found that because the Doppler shifted light from the retina performs a retro-illumination of the anterior segment, LDH reveals the structures that absorb or diffuse light in the cornea and eye lens. The various types of cataract could be characterized, and in most cases had little to moderate influence on Doppler fundus imaging. Finally, we also used the ability to holographically image simultaneously the anterior and posterior segments of the eye to track the movements of the retina and pupil with high temporal resolution.

Conclusions : In conclusion, we show that on top of its ability to image retinal and choroidal blood flow, LDH is also able to jointly perform blood flow imaging in the anterior segment, to track the eye movements, and to assess the lens and corneal transparency.

This is a 2021 Imaging in the Eye Conference abstract.

 

LDH imaging of a posterior subcapsular cataract.

LDH imaging of a posterior subcapsular cataract.

 

LDH imaging of a corneal neovascularization and scarring following an herpetic ulcer.

LDH imaging of a corneal neovascularization and scarring following an herpetic ulcer.

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