August 2021
Volume 62, Issue 11
Open Access
ARVO Imaging in the Eye Conference Abstract  |   August 2021
Measurement Repeatability of Sublayers of the Retinal Pigment Epithelium (RPE) using Visible Light Optical Coherence Tomography (vis-OCT)
Author Affiliations & Notes
  • Zeinab Ghassabi
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Roman V. Kuranov
    Opticent Inc., Evanston, Illinois, United States
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Mengfei Wu
    Ophthalmology, NYU Langone Health, New York, New York, United States
    Population Health and Environmental Medicine, New York University Division of Biostatistics, New York, New York, United States
  • Behnam Tayebi
    Ophthalmology, NYU Langone Health, New York, New York, United States
    Neuroscience Institute, NYU Langone Health, New York, New York, United States
  • Samantha Palmer
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Johnny Li
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Ronald Zambrano
    Ophthalmology, NYU Langone Health, New York, New York, United States
  • Ian Rubinoff
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Yuanbo Wang
    Opticent Inc., Evanston, Illinois, United States
  • Gadi Wollstein
    Ophthalmology, NYU Langone Health, New York, New York, United States
    Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, New York, United States
  • Joel Schuman
    Ophthalmology, NYU Langone Health, New York, New York, United States
    Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, New York, United States
  • Hao F. Zhang
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
    Opticent Inc., Evanston, Illinois, United States
  • Hiroshi Ishikawa
    Ophthalmology, NYU Langone Health, New York, New York, United States
    Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, New York, United States
  • Footnotes
    Commercial Relationships   Zeinab Ghassabi, None; Roman V. Kuranov, None; Mengfei Mengfei.Wu@nyulangone.org, None; Behnam Tayebi, None; Samantha Palmer, None; Johnny Li, None; Ronald Zambrano, None; Ian Rubinoff, None; Yuanbo Wang, None; Gadi Wollstein, None; Joel Schuman, Zeiss (P); Hao F. Zhang, Opticent Inc (P), Personal Financial Interest (I); Hiroshi Ishikawa, None
  • Footnotes
    Support  NIH: R01-EY013178, P30EY013079, unrestricted grant from Research to Prevent Blindness
Investigative Ophthalmology & Visual Science August 2021, Vol.62, 58. doi:
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      Zeinab Ghassabi, Roman V. Kuranov, Mengfei Wu, Behnam Tayebi, Samantha Palmer, Johnny Li, Ronald Zambrano, Ian Rubinoff, Yuanbo Wang, Gadi Wollstein, Joel Schuman, Hao F. Zhang, Hiroshi Ishikawa; Measurement Repeatability of Sublayers of the Retinal Pigment Epithelium (RPE) using Visible Light Optical Coherence Tomography (vis-OCT). Invest. Ophthalmol. Vis. Sci. 2021;62(11):58.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Multiple sublayers of retinal pigment epithelium (RPE) can be visualized with vis-OCT. Although the detailed anatomical interpretation has not been established yet, consistent measurements of such structures may have a potential to provide clinically useful biomarkers. The purpose of this study was to assess the RPE sublayer thickness measurement repeatability using vis-OCT.

Methods : Nine eyes of nine healthy volunteers were enrolled and scanned three times at the initial visit. Four of them were scanned another set of three scans on the following visits. Raster speckle-reduction scans (3×3×1.2 mm3 sampled with 512×8×1024 voxels in x, y, and z-axis, respectively) were acquired centering at the superior macular region using our prototype Aurora X1 vis-OCT system (Opticent Inc., Evanston, IL). For thickness measurements, four borders delineating the 3 bright RPE sublayers were manually segmented using averaged 15 A-line profile (Figure). Coefficient of variations (CVs) were calculated to assess the measurement repeatability for each of 3 RPE sublayers thickness.

Results : Mean age of the subjects were 44.9 ± 12.7 years old, with visual field mean deviation (MD) range from -1.6 to 1.1 dB, and global mean circumpapillary retinal nerve fiber layer (RNFL) thickness of 97.8 ± 12.5 (um). All sublayers showed high repeatability on both intra- and inter-session assessment (all CVs < 8%) (Table).

Conclusions : Vis-OCT allows us for visualization and quantification of RPE sublayers with high repeatability. RPE sublayer assessment using vis-OCT may provide clinically useful information on eyes with ocular diseases affecting RPE morphology.

This is a 2021 Imaging in the Eye Conference abstract.

 

Figure. RPE Visualization) a) Healthy subject‘s Bscan demonstrating RPE sublayers b) the corresponding average of 15 A-scans from the highlighted location.

Figure. RPE Visualization) a) Healthy subject‘s Bscan demonstrating RPE sublayers b) the corresponding average of 15 A-scans from the highlighted location.

 

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