August 2021
Volume 62, Issue 11
Open Access
ARVO Imaging in the Eye Conference Abstract  |   August 2021
Entry of cerebrospinal fluid components into the anterior chamber of the eye
Author Affiliations & Notes
  • Muneeb A Faiq
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Vishnu Adi
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Sophia Khoja
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Anoop Sainulabdeen
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Russell W Chan
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Carlos Parra
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Giles Hamilton-Fletcher
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
  • Choong H Lee
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Jiangyang Zhang
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Gadi Wollstein
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, New York University, New York, New York, United States
  • Joel Schuman
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Biomedical Engineering, NYU Tandon School of Engineering, New York University, New York, New York, United States
  • Kevin C Chan
    Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York, United States
    Department of Radiology, New York University Grossman School of Medicine, New York, New York, United States
  • Footnotes
    Commercial Relationships   Muneeb Faiq, None; Vishnu Adi, None; Sophia Khoja, None; Anoop Sainulabdeen, None; Russell Chan, None; Carlos Parra, None; Giles Hamilton-Fletcher, None; Choong Lee, None; Jiangyang Zhang, None; Gadi Wollstein, None; Joel Schuman, None; Kevin Chan, None
  • Footnotes
    Support  This work was supported in part by the National Institutes of Health R01-EY028125, P30-CA016087 and P41-EB017183 (Bethesda, Maryland); BrightFocus Foundation G2019103 (Clarksburg, Maryland); Feldstein Medical Foundation Research Grant (Clifton, New Jersey); Research to Prevent Blindness/Stavros Niarchos Foundation International Research Collaborators Award (New York, New York); and an unrestricted grant from Research to Prevent Blindness to NYU Langone Health Department of Ophthalmology (New York, New York).
Investigative Ophthalmology & Visual Science August 2021, Vol.62, 37. doi:
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    • Get Citation

      Muneeb A Faiq, Vishnu Adi, Sophia Khoja, Anoop Sainulabdeen, Russell W Chan, Carlos Parra, Giles Hamilton-Fletcher, Choong H Lee, Jiangyang Zhang, Gadi Wollstein, Joel Schuman, Kevin C Chan; Entry of cerebrospinal fluid components into the anterior chamber of the eye. Invest. Ophthalmol. Vis. Sci. 2021;62(11):37.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Recent evidence suggested the close interplay between intraocular pressure and intracranial pressure in health and disease, yet whether and how cerebrospinal fluid (CSF) communicates with the eye remains unclear. Here, we used in vivo contrast-enhanced MRI (CE-MRI) to investigate the entry of CSF components into the eye.

Methods : A polyethylene tubing was placed intrathecally at the lumbar region (L4-L5) of 34 healthy adult C57BL/6J mice followed by CE-MRI using a 7-Tesla Bruker scanner. Group 1 (n=20) received Gd-DTPA (molecular weight=0.9kDa) as contrast agent and Group 2 (n=14) received GadoSpin (molecular weight=200kDa). During MRI, animals were maintained under 1.0%-1.5% isoflurane at 37±1 oC rectal temperature. Contrast dynamics was monitored using a 3D T1-weighted sequence with 78x78x78 µm3 isotropic resolution. Twelve continuous scans (each lasting 10 min) were performed with 3 baseline acquisitions followed by 30 min of contrast infusion into the CSF space via the catheter while scanning continued until the 12th timepoint. T1-weighted contrast intensity (CI) changes over time were quantified in 7 regions of interest (ROIs) and compared within and between groups. These ROIs included posterior optic nerve subarachnoid space (ONSAS-P) distal to the eye, anterior ONSAS (ONSAS-A) proximal to the eye, olfactory bulb (OB), muscle tissue (MT), vitreous, lens, and anterior chamber (AC) (Fig. 1).

Results : Between-group ANOVAs were significant for ONSAS-P, ONSAS-A, OB, and AC (all p’s<.001), and NS for other ROIs. Bonferroni-corrected independent t-tests comparing each ROI between groups revealed that Group 1 had significantly higher CI than Group 2 for ONSAS-P [t(27.72)=13.05, p<.001, Mean difference (Mdiff)=474.27, d=4.27], ONSAS-A [t(32)=6.12, p<.001, Mdiff=146.15, d=2.11], OB [t(32)=5.96, p<.001, Mdiff=194.98, d=2.17] and AC [t(24.25)=4.02, p=.004, Mdiff=217.25, d=1.30] respectively. Time-to-peak was significantly different between groups for ONSAS-P [p<.001, Mdiff=29.71], ONSAS-A [p<.001, Mdiff=29.43], and AC [p=0.004, Mdiff=-8.21] (Fig. 2).

Conclusions : CSF components can enter the AC and possibly affect the composition of aqueous humor in a molecular size-dependent manner. Further studies are required to probe the pathways of this CSF-aqueous communication and its role in central and ocular physiology and diseases.

This is a 2021 Imaging in the Eye Conference abstract.

 

 

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