June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Starbust amacrine cells impairment in a rat model of chronic ocular hypertension.
Author Affiliations & Notes
  • Maria D Pinazo-Duran
    Surgery-Ophthalmology Department at the Faculty of Medicine, Ophthalmic Research Unit Santiago Grisolia, VALENCIA, Valencia, Spain
  • Agustina Noailles
    Departmet of physiology, genetics and microbiology, Departmet of physiology, genetics and microbiology, Alicante, Alicante, Spain
  • Aloma Mayordomo-febrer
    Veterinary, CEU Universidad Cardenal Herrera Servicio de Idiomas, Valencia, Valencia, Spain
  • Silvia Maria Sanz-Gonzalez
    Surgery-Ophthalmology Department at the Faculty of Medicine, Ophthalmic Research Unit Santiago Grisolia, VALENCIA, Valencia, Spain
  • Pedro Lax
    Departmet of physiology, genetics and microbiology, Departmet of physiology, genetics and microbiology, Alicante, Alicante, Spain
  • Maria del Mar Lopez-Murcia
    Veterinary, CEU Universidad Cardenal Herrera Servicio de Idiomas, Valencia, Valencia, Spain
  • oksana kutsir
    Departmet of physiology, genetics and microbiology, Departmet of physiology, genetics and microbiology, Alicante, Alicante, Spain
  • Nicolas Cuenca
    Departmet of physiology, genetics and microbiology, Departmet of physiology, genetics and microbiology, Alicante, Alicante, Spain
  • Footnotes
    Commercial Relationships   Maria Pinazo-Duran None; Agustina Noailles None; Aloma Mayordomo-febrer None; Silvia Maria Sanz-Gonzalez None; Pedro Lax None; Maria del Mar Lopez-Murcia None; oksana kutsir None; Nicolas Cuenca None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 935 – A0404. doi:
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      Maria D Pinazo-Duran, Agustina Noailles, Aloma Mayordomo-febrer, Silvia Maria Sanz-Gonzalez, Pedro Lax, Maria del Mar Lopez-Murcia, oksana kutsir, Nicolas Cuenca; Starbust amacrine cells impairment in a rat model of chronic ocular hypertension.. Invest. Ophthalmol. Vis. Sci. 2022;63(7):935 – A0404.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the retinal starbust amacrine cells (SBACs) by morphological and morphometrical approaches, in a rat model of chronic ocular hypertension (OHT).

Methods : Weekly intracameral injections of sodium hyaluronate (HYA) were used to induce chronic OHT in the left eye (LE), whilst salt solution was injected in the sham operated right eyes (RE) of seven male Wistar rats, during nine consecutive weeks. Intraocular pressure (IOP) measurements were done weekly, by means of a rebound tonometer. Retinal cryosections were obtained for immunohistochemical techniques (specifically ChAT antibody to SBACs), and morphometric approaches. Statistics were performed by the 28.0 SPSS program.

Results : The OHT was significantly higher and sustained during the follow-up in LE respect to RE at most timepoints. Considering that in the retinal ganglion cell (RGC) layer, the displaced amacrine cells (DACs) constituted up to 50% of all cells, and the SBACs account for 20% of the DACs population, mild-to-moderate OHT induced noticeable morphologic changes and a significant reduction in ON/OFF SBACs in the HYA-treated eyes (17.7 ± 2.9 cells/mm) with respect to the controls (24.8 ± 3.1 cells/mm), (p<0.01), contributing to the RGC layer global reduction.

Conclusions : The HYA intracameral injections in the rat eye induced a mild-to-moderate sustained IOP elevation and RGC loss, mimicking most human open-angle glaucoma milestones. The decreased density of SBACs and its deteriorated ON/OFF plexus in our chronic OHT model, probably started the fall off in the signal transduction and visual performance, that for the first time, may be related to specific anomalies in motion perception described in glaucoma patients.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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