June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
A novel approach to analyze white noise ERGs in mice
Author Affiliations & Notes
  • Nina Stallwitz
    Department of Ophthalmology, Section for Retinal Physiology, University Hospital Erlangen, Erlangen, Bayern, Germany
    Biology, Section for Animal Physiology, Friedrich-Alexander-Universitat Erlangen-Nurnberg Naturwissenschaftliche Fakultat, Erlangen, Bayern, Germany
  • Anneka Joachimsthaler
    Department of Ophthalmology, Section for Retinal Physiology, University Hospital Erlangen, Erlangen, Bayern, Germany
    Biology, Section for Animal Physiology, Friedrich-Alexander-Universitat Erlangen-Nurnberg Naturwissenschaftliche Fakultat, Erlangen, Bayern, Germany
  • Jan J Kremers
    Department of Ophthalmology, Section for Retinal Physiology, University Hospital Erlangen, Erlangen, Bayern, Germany
  • Footnotes
    Commercial Relationships   Nina Stallwitz None; Anneka Joachimsthaler None; Jan Kremers None
  • Footnotes
    Support  DFG Grant KR1317/17-1
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 749 – F0401. doi:
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    • Get Citation

      Nina Stallwitz, Anneka Joachimsthaler, Jan J Kremers; A novel approach to analyze white noise ERGs in mice. Invest. Ophthalmol. Vis. Sci. 2022;63(7):749 – F0401.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To analyse the correlation between ERG response to temporal white noise (TWN) stimuli while modulating luminance or single photoreceptor types.

Methods : Mice, with human L-opsin instead of the murine M-opsin were dark adapted overnight, all further handling was done under dim red light. Recordings of the anesthetized animals were performed with the Celeris Ganzfeld stimulator. Measurements were performed at different mean luminances (MLs). The animals adapted to a new mean luminance for 1 min prior to recordings.
ERGs to TWN stimuli (containing all frequencies up to 20 Hz with equal amplitudes and random phases) were recorded. The stimuli were luminance modulation (100% contrast white light) at MLs ranging from −0.7 to 1.1 log cd/m2. In addition, photoreceptor isolating stimuli using the silent substitution method (52% rod-contrast, 48% L-Cone contrast, 77% S-Cone contrast) were employed with MLs between −0.8 and 1.0 log cd/m2.
Each recording was performed twice. To study the reproducibility of the recordings, ERG potentials in the two recordings at identical instances during stimulation were plotted against each other. The square of the correlation coefficient (r2repr) of the linear regression through the data quantified the correlation between the two.
To see how well preserved the distinct peaks of the wnERG were at different MLs, the ERG potentials at all MLs were plotted against those to the highest ML (for the cones) and at the lowest ML (for the rods) at identical instant during recording. The linear regressions and the r2 (r2ML) were used to quantify the correlations between them.

Results : For luminance stimuli r2repr values decreased with increasing ML to −0.1 log cd/m2 ML above which r2repr increased. For rod isolating stimuli r2repr values decreased with increasing MLs; for cone isolating stimuli they were similar at all MLs.
R2ML values for luminance and L- and S-opsin stimuli increased with increasing ML, whereas they decreased for rods.

Conclusions : The differences between the characteristics of the r2 values between rods and cones point at fundamental differences of the dynamics of the ERG origins. Origins of luminance ERGs are a mixture and depend on ML.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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