June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Pilot investigation of multimodal and multiscale retinal imaging technology developed by a trans-European multidisciplinary consortium
Author Affiliations & Notes
  • Kiyoko Gocho
    Clinical Investigation Center 1423, Inserm, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Nicolas Lefaudeux
    Imagine eyes, Orsay, France
  • Claudia Valdes
    Barcelona Institute of Science and Technology, Institut de Ciencies Fotoniques, Barcelona, Spain
  • Jordi Andilla
    Barcelona Institute of Science and Technology, Institut de Ciencies Fotoniques, Barcelona, Spain
  • Muhammad Faizan Shirazi
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Florian Schwarzhans
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Konstantinos Ntatsis
    Biomedical Imaging Group Rotterdam, Department of Radiology and Nuclear Medicine, Erasmus MC, Rotterdam, Zuid-Holland, Netherlands
  • Luisa Sanchez Brea
    Biomedical Imaging Group Rotterdam, Department of Radiology and Nuclear Medicine, Erasmus MC, Rotterdam, Zuid-Holland, Netherlands
  • Danilo Andrade De Jesus
    Biomedical Imaging Group Rotterdam, Department of Radiology and Nuclear Medicine, Erasmus MC, Rotterdam, Zuid-Holland, Netherlands
  • Marie Elise Wistrup Torm
    Department of Ophthalmology & University of Copenhagen, Rigshospitalet, Kobenhavn, Denmark
  • Christina Eckmann-Hansen
    Department of Ophthalmology & University of Copenhagen, Rigshospitalet, Kobenhavn, Denmark
  • Abdullah Amini
    Department of Ophthalmology & University of Copenhagen, Rigshospitalet, Kobenhavn, Denmark
  • Kate Grieve
    Clinical Investigation Center 1423, Inserm, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Pablo Loza-Alvarez
    Barcelona Institute of Science and Technology, Institut de Ciencies Fotoniques, Barcelona, Spain
  • Michael Pircher
    Center for Medical Physics and Biomedical Engineering, Medizinische Universitat Wien, Wien, Wien, Austria
  • Michel Paques
    Clinical Investigation Center 1423, Inserm, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, Île-de-France, France
  • Footnotes
    Commercial Relationships   Kiyoko Gocho None; Nicolas Lefaudeux Imagine eyes, Code E (Employment); Claudia Valdes None; Jordi Andilla None; Muhammad Shirazi None; Florian Schwarzhans None; Konstantinos Ntatsis None; Luisa Sanchez Brea None; Danilo Andrade De Jesus None; Marie Elise Torm None; Christina Eckmann-Hansen None; Abdullah Amini None; Kate Grieve None; Pablo Loza-Alvarez None; Michael Pircher None; Michel Paques None
  • Footnotes
    Support  Horizon 2020, the European Union’s Framework Program for Research and Innovation under grant agreement no. 780989 – MERLIN
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 1480. doi:
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      Kiyoko Gocho, Nicolas Lefaudeux, Claudia Valdes, Jordi Andilla, Muhammad Faizan Shirazi, Florian Schwarzhans, Konstantinos Ntatsis, Luisa Sanchez Brea, Danilo Andrade De Jesus, Marie Elise Wistrup Torm, Christina Eckmann-Hansen, Abdullah Amini, Kate Grieve, Pablo Loza-Alvarez, Michael Pircher, Michel Paques; Pilot investigation of multimodal and multiscale retinal imaging technology developed by a trans-European multidisciplinary consortium. Invest. Ophthalmol. Vis. Sci. 2022;63(7):1480.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Adaptive optics (AO) has enabled visualizing cellular structure in the retina, yet its clinical usability has been hampered by small field-of-view and bulkiness of instrumentation. We developed a compact imaging system with a multiscale optical architecture that delivers large views of the retina as well as highly-magnified AO-corrected images. This study explored imaging performance in human eyes.

Methods : The prototype included three imaging modalities: confocal scanning laser ophthalmoscopy (SLO) based on 786 nm super-luminescent diode scanned at 8 kHz, optical coherence tomography (OCT) based on a 1050 nm swept-source at 200 kHz, and OCT angiography (OCTA). Each modality could be operated with a field-of-view of either 40 x 30 deg or 4 x 3 deg. In the latter case, ocular wavefront aberrations were corrected by AO operating in closed loop at 13 Hz. During OCT/OCTA examinations, head and eye movements were compensated by automated pupil and retinal tracking at 100 Hz. The ability to visualize microscopic retinal structure was experimented in 35 healthy volunteers and 39 patients affected by various fundus pathologies, including diabetic retinopathy, age-related macular degeneration and inherited retinal dystrophies.

Results : The overall dimensions of the system were 49 x 50 x 50 cm. Large-field SLO and OCT provided quick overviews of the retina while both AOSLO and AOOCT enabled the visualization of parafoveal cone cells, nerve fiber bundles, capillaries, and microscopic lesions. Multiple-averaged AOOCT B-scans revealed Müller-cell-like structures. AOOCTA could image capillaries and choriocapillaris with minimal shadowing artifacts. In pathological cases, AOSLO showed the distribution and lateral extent of microscopic lesions like micro-aneurisms, micro-exudates, atrophic region borders, pigment clusters, and photoreceptor losses. AOOCT scans completed these observations with information on depth location and axial extent of these alterations.

Conclusions : The multimodal multiscale imaging architecture enabled in-depth retinal examinations through a straightforward process: 1) acquire an overview image, 2) identify regions of interest, 3) examine these regions with near-histology 3-D resolution. When confirmed by further investigations, this technology should offer new possibilities for retinal phenotyping and clinical research in new therapies.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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