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SRIKANTH DUMPATI, Sunil Shah, Shehzad Naroo, Debarun Dutta; Antimicrobial efficacy of novel Ultraviolet- C device against microorganisms related to Contact lens keratitis.. Invest. Ophthalmol. Vis. Sci. 2022;63(7):1466.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the antimicrobial activity of a novel ultraviolet-C device against microorganisms implicated in contact lens related adverse events.
The Ultraviolet-C device with 265nm emitting LED was used. Delfilicon-A (Dailies Total-1), Senofilicon-A (Acuvue OASYS), Comfilicon-A (Biofinity), Balafilicon-A (Purevison 2), Samfilicon- A(Ultra) silicone hydrogels, Omafilicon-A (Proclear) hydrogel contact lens materials and Lens cases (B&L) were exposed to Staphylococcus aureus 38, Pseudomonas aeruginosa 6294, Candida albicans ATCC 76615 and Fusarium Solani 10696 with overnight incubation at 37°C followed by 30 seconds exposure to UVC from 8 mm distance. After this, lenses were gently washed with phosphate buffer saline and were plated on nutrient agar plates for determination of viable count. Viable bacteria were enumerated as CFU/lens and CFU/lens case.
Exposure to ultraviolet rays by a novel UVC device statistically significantly (P<0.05) reduced mirobial contamination of contact lens and lens cases. Exposure to UVC device induced 2.15±1.52, 3.15±0.92, 3.27±0.19, 2.80±0.64, 3.54±0.82, 3.15±0.28 and 4.69±0.04 log reduction against S. aureus 38, 0.90±1.28, 3.01±0.85, 1.72±0.43, 2.56±0.56, 3.54±0.35, 2.28±1.06 and 4.79±0.54 log reduction against P. aeruginosa 6294 ,1.55±1.07, 2.09±0.30, 0.72±0.25, 1.66±1.47, 1.66±0.13, 1.32±0.16, and 3.76±0.13 log reduction against C.albicans ATCC 76615, 4.18±0.07, 2.16±0.32, 4.03±0.50, 3.34±0.77, 2.16±0.32, 2.16±0.32 and 3.60±0.35 log reduction against F.Solani 10696 on Delfilicon-A, Senofilcon-A, Comfilicon-A, Omafilicon- A, Balafilicon-A, Samfilicon-A lenses and lens cases respectively.
Ultraviolet-C has high antimicrobial efficacy against most of the predominant microorganisms implicated in contact lens related keratitis and it could be readily used as a broad-spectrum antimicrobial treatment.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.
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