June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Dual patch-clamp recording of synaptic transmission between morphologically and physiologically characterized cone bipolar cell types and a diffused ganglion cell in wholemount mouse retina
Author Affiliations & Notes
  • Yao Xue
    Yale University Department of Ophthalmology and Visual Science, New Haven, Connecticut, United States
    Yale University Graduate School of Arts and Sciences, New Haven, Connecticut, United States
  • Seunghoon Lee
    Yale University Department of Ophthalmology and Visual Science, New Haven, Connecticut, United States
  • Jimmy Zhou
    Yale University Department of Ophthalmology and Visual Science, New Haven, Connecticut, United States
  • Footnotes
    Commercial Relationships   Yao Xue None; Seunghoon Lee None; Jimmy Zhou None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 1164. doi:
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      Yao Xue, Seunghoon Lee, Jimmy Zhou; Dual patch-clamp recording of synaptic transmission between morphologically and physiologically characterized cone bipolar cell types and a diffused ganglion cell in wholemount mouse retina. Invest. Ophthalmol. Vis. Sci. 2022;63(7):1164.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : While bipolar cells have been successfully studied with imaging, connectomic, and transcriptomic methods in recent years, the output synaptic physiology and functional circuitry of bipolar cell types remain poorly understood, except in the case of rod bipolar cells. This study is to characterize the physiology and synaptic transmission of identified cone bipolar cell (CBC) types in the mouse retina.

Methods : Patch-clamp recordings were made with two-photon microscopy from various morphological types of CBCs in the wholemount mouse retina. Receptive field properties were characterized using visual stimuli of various sizes and patterns. Dual patch-clamp recording was used to characterized synaptic transmission from CBC types to an ON-OFF small-field ganglion cell (tentatively termed XGC), which was brightly labelled in a transgenic line used.

Results : Receptive-field properties, baseline synaptic inputs, and major voltage-gated currents were characterized for 12 out of the 14 known morphological types of CBCs in the wholemount mouse retina. A catalog was generated to correlate physiological properties with morphological types of CBCs. Almost every CBC type showed distinctive physiological characteristics that distinguished one type from other types of CBCs. When the physiological and morphological features were analyzed together, all CBC types could be unambiguously defined. Dual patch-clamp recordings were then made from pairs of physiologically and morphological identified CBC types and XGCs in the whole-mount retina. Two distinctive kinetic patterns of signal transmission were found, suggesting differential temporal encoding of spatial visual integration. The results revealed an intriguing synaptic organization involving both chemical and electric synapses.

Conclusions : This study correlated distinct receptive-field and intrinsic physiological properties with morphologically identified CBC types and revealed new patterns of synaptic transmission from CBC types to XGCs, which may enhance spatiotemporal encoding of visual information.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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