Abstract
Purpose :
To study the effects and the underlying mechanism of latanoprost on induced myopia in chicken
Methods :
Myopia was induced in the right eyes of chicks using diffuser lenses (FDM) or -10D single vision lenses (LIM) for 12 days, while the left eyes were untreated control. One drop of topical latanoprost 0.005% or saline was administered daily in the treated and control groups, respectively. Refractive errors, tonometry and high-frequency A-scan ultrasonography were measured on days 0, 4, 8 and 12. Eyeballs were enucleated and processed for hematoxylin & eosin staining and western blot respectively. A total of 40 chicks were randomly divided into four groups (n=10). Data are presented as mean interocular differences ± standard deviation (SD).
Results :
After 12 days of treatment, latanoprost inhibited both FDM and LIM. FDM control: -16.02±2.80D vs FDM latanoprost: -10.86±2.54D, p<0.0001. LIM control: -10.90±1.37D vs LIM latanoprost: -6.62±1.39D, p<0.0001) respectively. Intraocular pressure (IOP) was increased in FDM and LIM groups applied with saline, but decreased in those applied with latanoprost. Histology showed that latanoprost inhibited the thinning of sclera in the FDM and LIM groups. Latanoprost also upregulated retinal Early Growth Response 1 (Egr1) protein expression at the 12-day time point.
Conclusions :
Topical latanoprost inhibit FDM and LIM in the chicken model and its effect was associated with a reduced IOP and upregulated Egr1 protein expression.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.