June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Large-Area RPE Removal by Microsecond Laser followed by hiPS-RPE transplantation
Author Affiliations & Notes
  • Boris V. Stanzel
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Eye Clinic Sulzbach, Sulzbach, Saarland, Germany
  • Christian Burri
    Institute for Human Centered Engineering - optoLab, Berner Fachhochschule, Bern, Bern, Switzerland
    Institute of Applied Physics, Universitat Bern, Bern, Bern, Switzerland
  • Sami Al-Nawaiseh
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Ophthalmology, University of Muenster, Muenster, North-Rhine Westfalia, Germany
  • Philip Wakili
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Eye Clinic Sulzbach, Sulzbach, Saarland, Germany
  • Sylvia Jane Gasparini
    Center for Regenerative Therapies Dresden, Technische Universitat Dresden, Dresden, Sachsen, Germany
  • Gerardo Farese
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Eye Clinic Sulzbach, Sulzbach, Saarland, Germany
  • Christina Kroetz
    Fraunhofer IBMT, Germany
  • Boris Greber
    Catalent Pharma Solutions, Germany
  • Christoph Meier
    Institute for Human Centered Engineering - optoLab, Berner Fachhochschule, Bern, Bern, Switzerland
  • Martin Frenz
    Institute of Applied Physics, Universitat Bern, Bern, Bern, Switzerland
  • Peter Szurman
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Eye Clinic Sulzbach, Sulzbach, Saarland, Germany
  • André Schulz
    Klaus Heimann Eye Research Institute, Sulzbach, Saarland, Germany
    Eye Clinic Sulzbach, Sulzbach, Saarland, Germany
  • Marius Ader
    Center for Regenerative Therapies Dresden, Technische Universitat Dresden, Dresden, Sachsen, Germany
  • Footnotes
    Commercial Relationships   Boris Stanzel Geuder, Novartis, Apellis, Code C (Consultant/Contractor), Geuder, Catalent, Vitreq, MedOne Surgical, Code F (Financial Support), Bayer, Iridex, Heidelberg Engineering, Geuder, Code R (Recipient); Christian Burri Meridian AG, Heidelberg Engineering, Code F (Financial Support), Heidelberg Engineering, Meridian AG, Haag Streit, Code R (Recipient); Sami Al-Nawaiseh Heidelberg Engineering, Code R (Recipient); Philip Wakili None; Sylvia Gasparini None; Gerardo Farese None; Christina Kroetz None; Boris Greber Catalent, Code E (Employment); Christoph Meier None; Martin Frenz None; Peter Szurman Geuder, DORC, Code C (Consultant/Contractor), Novartis, Bayer, Code R (Recipient); André Schulz None; Marius Ader None
  • Footnotes
    Support  ReSight consortium (BMBF 01EK1613A)
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 1858. doi:
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      Boris V. Stanzel, Christian Burri, Sami Al-Nawaiseh, Philip Wakili, Sylvia Jane Gasparini, Gerardo Farese, Christina Kroetz, Boris Greber, Christoph Meier, Martin Frenz, Peter Szurman, André Schulz, Marius Ader; Large-Area RPE Removal by Microsecond Laser followed by hiPS-RPE transplantation. Invest. Ophthalmol. Vis. Sci. 2022;63(7):1858.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Cell therapeutics for AMD were often implanted regardless of RPE status in the target zone. This may result in RPE multilayering. Here we study a novel laser to remove RPE without collateral damage prior to RPE implantation to encourage better subretinal integration.

Methods : Pigment rabbits (n=24) were immunosuppressed with Sirolimus, Doxycyclin and Minocyclin. Using a SLO/ OCT (Heidelberg Engineering) extended with a prototype laser (Meridian Medical; wavelength: 532 nm; pulse duration, 8 µs), a large area of RPE was selectively removed in 19 rabbits. Animals without laser lesions served as controls (n=5). A 25 gauge vitrectomy (Geuder) with removal of posterior hyaloid membrane was performed thereafter. Human iPS-RPE (1000 cells/ µl) were manually injected using a 100 µl syringe (Hamilton) connected to a 38G cannula (MedOne) into the RPE laser lesion, or over healthy RPE in controls, monitored by intraoperative OCT imaging (RESCAN 700, Zeiss). In vivo follow up/ retinal imaging was up to 12 weeks including fluorescein and indocyanine angiography, as well as SD-OCT (Spectralis ®, Heidelberg Engineering).

Results : Representative RPE laser wounds exhibited mild late phase FA& ICGA leakage, without abnormal outer retinal or choroidal hyperreflectivity on OCT. By contrast, lesions with earlier leakage on FA/ ICGA showed beam-sized outer retinal hyperreflectivity on OCT, suggesting coagulation. The size of the RPE wounds was typically 10-12mm2.
iOCT demonstrated in an immediate and directed spread of the bleb retinal detachment (bRD) within the lasered zone. By contrast, bRDs performed over non-lasered RPE raised slower with a circular spread. Subretinal injection ranged from 5-70µl, with lesser volumes/ larger bRDs areas over lasered regions.
At 6 and 12 weeks, none of implanted regions showed FA/ICGA leakage, some lesions had blockage due to hyperpigmentation; on OCT, representative areas showed preserved ellipsoid bands, with some RPE undulations. Lasered/implanted areas with a peripheral hyperpigmentation showed central outer retinal atrophy along with irregular RPE. Control implantation sites showed retinal atrophy and a variably thickened RPE band.

Conclusions : Large-area RPE removal with laser disruption is feasible in healthy rabbits and appears to facilitate superior integration of RPE suspension grafts, compared to subretinal injection alone. Future work aims to correlate histology with in vivo imaging.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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