Abstract
Purpose :
To evaluate repeatability, reproducibility, and accordance between NITBUT, tear meniscus and meibography measurements within three different ocular surface imaging devices.
Methods :
We performed an observational study on 66 healthy eyes. Tear meniscus, NITBUT (non-invasive tear break-up time) and meibography were measured using 3 types of corneal imaging devices: Keratograph 5M (Oculus, Wetzlar, Germany), Antares (Lumenis, Sidney, Australia) and LacryDiag (Quantel Medical, Cournon d’Auvergne, France). One-way ANOVAs and Welch ANOVAs were used to calculate correlation. Reliability and accordance between the tear meniscus and NITBUT were defined using Tukey Honest Significant Differences, Bonferroni corrections and plotted in Tukey mean difference plots. Accordance from meibography classification was analyzed by calculating Fleiss' Kappa Index and presented visually in Venn diagrams.
Results :
We observed discordance between measurements of tear meniscus height between the three devices, F2, 195= 15.24, p<0.01. Measurements performed with Antares were higher; 0.365±0.0851, than those with both the Keratograph5M and LacryDiag; 0.293±0.0790 and 0.306±0.0731. NITBUT measurements also showed discordance between devices, F2,111 = 13.152, p < 0.01. Measurements performed with LacryDiag were lower (10.4±1.82) compared to those obtained with Keratograph5M (12.6±4.01) and Antares (12.6±4.21). Fleiss' Kappa coefficient showed a value of -0.00487 for the upper lid and 0.128 for the inferior lid Meibography classification, suggesting discrete to poor agreement between measurements.
Conclusions :
Depending on the device used and the parameter analyzed, measurements varied between each other, showing difference in image processing.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.