June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Evaluating the outcomes of dried vs cryopreserved amniotic membrane grafting in the treatment of corneal epithelial defects
Author Affiliations & Notes
  • James Tian
    Duke University Department of Ophthalmology, Durham, North Carolina, United States
  • Clayton Wisely
    Duke University Department of Ophthalmology, Durham, North Carolina, United States
  • Footnotes
    Commercial Relationships   James Tian None; Clayton Wisely None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 3260 – A0295. doi:
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      James Tian, Clayton Wisely; Evaluating the outcomes of dried vs cryopreserved amniotic membrane grafting in the treatment of corneal epithelial defects. Invest. Ophthalmol. Vis. Sci. 2022;63(7):3260 – A0295.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Amniotic membrane grafts (AMGs) have been used to provide nutritious and anti-inflammatory scaffolding to the cornea surface to promote epithelial wound healing. While initial AMGs were first prepared with cryotherapy, there are logistical advantages of using dried AMGs that can be stored at room temperature. However, there is insufficient clinical data on the outcomes of amniotic membrane grafting between dried vs cryopreserved AMGs for specific indications. Thus, we aim to compare the resolution rate of corneal epithelial defects when treated with a dried vs cryopreserved AMGs.

Methods : A search for CPT codes for AMG placement was performed in our institution’s electronic health record, and the corresponding charts were reviewed to isolate patients who received an AMG for a corneal epithelial defect. The type of (dried vs cryopreserved) AMG, presenting best-corrected visual acuity (BCVA), indications for AMG, and anterior segment exam data were collected. Patients were separated into a “fully healed”, “partially healed”, or “no improvement” based on clinical documentation. Statistics were computed with Chi-square and Student’s t-test.

Results : A total of 43 charts were reviewed of patients who underwent AMG placement for corneal epithelial defects. 32 patients underwent dried AMG placement, 11 underwent cryopreserved AMG placement. The indications ranged from persistent epithelial defects after corneal transplantation or superficial keratectomy to herpetic neurotrophic keratitis or ocular cicatricial pemphigoid. The mean age of the dried AMG group was 68 and the mean age of the cryopreserved group was 64. The mean presenting BCVA was higher in the dried AMG group (20/160-20/200) than in the cryopreserved AMG group (20/400-20/600). The rate of full or partial epithelial healing was 94% (30/32) for the dried AMG group and 91% (10/11) for the cryopreserved AMG group (p=0.75).

Conclusions : The logistically simpler dried AMG and the cryopreserved AMG showed similar outcomes when used to treat corneal epithelial defects.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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