Abstract
Purpose :
To determine the effect of three amniotic scaffolds (Biovance3L Ocular (Biovance3L), AMBIO2®, AmnioGraft®) on human ocular epithelial cell adhesion and proliferation.
Methods :
Human corneal epithelial cells (HCEC) and human conjunctival epithelial cells (HConEpiC) were seeded into wells. Adhesion and proliferation were measured at days 1, 4 and 7 on the scaffolds. Extracted conditioned media was used for growth assays.
Results :
Biovance3L showed significantly higher epithelial cell viability (P < 0.001) and significantly greater epithelial cell adhesion (P ≤ 0.011) vs other scaffolds. Epithelial cell proliferation rate was
significantly greater on Biovance3L than AmnioGraft® (P < 0.001). HCEC migration from cells cultured on Biovance3L and AMBIO2® were comparable (P = 0.885) and significantly greater than AmnioGraft® (P ≤ 0.006). The different scaffolds did not affect the migration of HConEpiC.
Conclusions :
Biovance3L had a significant effect on human epithelial cells by supporting greater viability, adhesion, and proliferation compared with other scaffolds. Further research is needed to assess the clinical impact of these findings.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.