June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Characterization of Rabbit Corneal Ulceration Model for Pre-Clinical Studies.
Author Affiliations & Notes
  • Anil Tiwari
    Ophthalmology-Cornea and Stem cells, Dr. Shroff's Charity Eye Hospital, New Delhi, Delhi, India
  • Aastha Singh
    Opthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, United States
  • Jyoti Rajput
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Prayag bellur
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Bharti Sangwan
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Parinita Agrawal
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Midhun Ben Thomas
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Shivaram Selvam
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Arun Chandru
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Tuhin Bhowmick
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Virender Singh Sangwan
    Ophthalmology-Cornea and Stem cells, Dr. Shroff's Charity Eye Hospital, New Delhi, Delhi, India
    Pandorum Technologies Pvt. Ltd., Bangalore, Karnataka, India
  • Footnotes
    Commercial Relationships   Anil Tiwari None; Aastha Singh None; Jyoti Rajput Pandorum Technologies Pvt. Ltd., Code E (Employment); Prayag bellur Pandorum technologies Pvt. Ltd., Code E (Employment); Bharti Sangwan Pandorum technologies Pvt. Ltd., Code E (Employment); Parinita Agrawal Pandorum technologies Pvt. Ltd., Code E (Employment); Midhun Ben Thomas Pandorum technologies Pvt. Ltd., Code E (Employment); Shivaram Selvam Pandorum technologies Pvt. Ltd., Code E (Employment); Arun Chandru Pandorum Technologies Pvt. Ltd. , Code P (Patent); Tuhin Bhowmick Pandorum technologies Pvt. Ltd., Code P (Patent); Virender Singh Sangwan Pandorum technologies Pvt. Ltd., Code P (Patent)
  • Footnotes
    Support  Ramalingaswami Fellowship, Department of Biotechnology
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 3642 – A0207. doi:
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      Anil Tiwari, Aastha Singh, Jyoti Rajput, Prayag bellur, Bharti Sangwan, Parinita Agrawal, Midhun Ben Thomas, Shivaram Selvam, Arun Chandru, Tuhin Bhowmick, Virender Singh Sangwan; Characterization of Rabbit Corneal Ulceration Model for Pre-Clinical Studies.. Invest. Ophthalmol. Vis. Sci. 2022;63(7):3642 – A0207.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Here we enumerate hallmarks of corneal fibrosis and ulceration: fibrosis and extracellular deposition (ECM), angiogenesis and inflammation. This study highlights the ophthalmological, clinical and histopathological changes throughout the progression of deep stromal alkali injury in the rabbit cornea.

Methods : Albino New Zealand rabbits were used for the study. Penetrating lamellar keratoplasty was performed on rabbit cornea (n=3) using guarded trephine. 0.75N NaOH was applied followed by irrigating with normal saline. Animals were imaged using the ophthalmological parameters; i) OCT, ii) Slit lamp and iii) Densitometry over the period of 3 weeks. At the end of three weeks animals were sacrificed and eyes were enucleated and further processed for histopathology (H&E, PAS staining) and Immunohistochemistry

Results : The parameters used in clinics for evaluating patients were used to evaluate and grade the scars into nebular, macular and leucomatous. Slit lamp reveled re-epithelization of the wound in first 5-6 days followed by incidences of epithelial defects, inflammation, and opacification. Neovascularization was observed after 14-15 days of alkali burn. OCT: pachymetry wide and raster scans reveled extensive edema and thickening of the central cornea. Densitometry images reveled stable scar formation with the opacity score ≥75, condition of legal blindness. Histology and immunohistochemistry evaluations supported the ophthalmic evaluation results.

Conclusions : The parameters used in clinics for evaluating patients were used to evaluate and grade the scars into nebular, macular and leucomatous. Slit lamp reveled re-epithelization of the wound in first 5-6 days followed by incidences of epithelial defects, inflammation, and opacification. Neovascularization was observed after 14-15 days of alkali burn. OCT: pachymetry wide and raster scans reveled extensive edema and thickening of the central cornea. Densitometry images reveled stable scar formation with the opacity score ≥75, condition of legal blindness. Histology and immunohistochemistry evaluations supported the ophthalmic evaluation results.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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