June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Participation of citrullinated histone a NET associated protein as inflammatory marker of diabetic retinopathy prognosis
Author Affiliations & Notes
  • Fátima Sofía Magaña Guerrero
    Cellular and Tissue Biology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Mexico city, Mexico, city , Mexico
  • Paola Sanchez-Cisneros
    Cellular and Tissue Biology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Mexico city, Mexico, city , Mexico
  • Jose Eduardo Aguayo-Flores
    Cellular and Tissue Biology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Mexico city, Mexico, city , Mexico
  • Beatriz Buentello-Volante
    Cellular and Tissue Biology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Mexico city, Mexico, city , Mexico
  • Lorenzo Islas-Vazquez
    Immunology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Mexico city, Mexico, city , Mexico
  • Jose Luis Rodriguez Loaiza
    Retina Department, Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, Mexico City, Mexico City, Mexico
  • Yonathan Garfias
    Medicine Faculty, Biochemistry Department, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico, Ciudad de México, Mexico
    Cellular and Tissue Biology Department, Instituto de Oftalmología Fundación Conde de Valenciana IAP, Instituto de Oftalmologia Fundacion Conde de Valenciana IAP, Mexico City, Mexico City, MX, other/health, Mexico,city, Mexico, city , Mexico
  • Footnotes
    Commercial Relationships   Fátima Magaña Guerrero None; Paola Sanchez-Cisneros None; Jose Eduardo Aguayo-Flores None; Beatriz Buentello-Volante None; Lorenzo Islas-Vazquez None; Jose Luis Rodriguez Loaiza None; Yonathan Garfias None
  • Footnotes
    Support  SECTEI 250/2019, PAPIIT: IN203821
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 3604 – A0059. doi:
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      Fátima Sofía Magaña Guerrero, Paola Sanchez-Cisneros, Jose Eduardo Aguayo-Flores, Beatriz Buentello-Volante, Lorenzo Islas-Vazquez, Jose Luis Rodriguez Loaiza, Yonathan Garfias; Participation of citrullinated histone a NET associated protein as inflammatory marker of diabetic retinopathy prognosis. Invest. Ophthalmol. Vis. Sci. 2022;63(7):3604 – A0059.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Diabetic retinopathy (DR) is the major vascular complication of diabetes and is the main cause of blindless worldwide. The development of DR is associated with chronic inflammation of diabetic patients. Neutrophils are inflammatory cells able to release structures formed by extracellular DNA decorated with proteolytic enzymes and proteins with posttranslational modifications such as citrullinated histone 3 (H3Citr) named neutrophil extracellular traps (NETs). The aim of this study is to determine the association between the serum levels of H3Citr and the DR in different clinical stages.

Methods : This is a cross-sectional study. Diabetic (DMT2) patients with and without different stages of RD were included as well as control subjects. Once the informed consent was signed, clinical, ophthalmological, and metabolic evaluations were performed, and a peripheral blood sample was obtained. Activation of Peripheral blood neutrophils (PBN) was achieved by means of flow cytometry, while H3Citr serum levels were evaluated by ELISA. H3Citr and metabolic status correlations were also performed. Finally, PBN-H3Citr was measured by means of western blot and compared among groups.

Results : CD66b is an activation marker for neutrophils. PBN-CD66b+ cells were significantly higher (p≤0.05) only in the Proliferative DR (PDR) group when compared between the other groups. We observed an increased concentration of H3Citr levels in DMT2 without DR [DMT2-non-DR], respect to the other groups. Regarding the association of H3Citr with the clinical and metabolic conditions, we found a significative positive correlation (p≤0.05) between serum concentration of H3Citr levels with their clinical and metabolic values such as Body Mass Index (BMI), glycosylated hemoglobin (HgA1b), fasting glucose, cholesterol, Low-Density Lipoproteins (LDL) and triglycerides (TG). Finally, H3Citr expression from PBN obtained from PRD and activated with ionomycin was significantly higher when compared with the other groups.

Conclusions : Taking together these results, suggest that PBN from PRD patients are more prone to be activated and this might reinforce the inflammatory hypothesis of the RD. Interestingly, serum H3Citr is able to predict a systemic inflammatory state among DMT2. This post translationally modified protein is of interest to study DMT2 and its main microvascular complication, the DR.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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