Abstract
Purpose :
To study the five common ocular antibiotics susceptibility of multidrug-resistant Staphylococcus isolated from conjunctival sac, cornea, eyelid margin and lacrimal sac in the ocular anterior segment.
Methods :
67 isolates were collected from patients from January 2018 to May 2019 at Henan Eye Hospital and identified by DL-STAPH Staphylococcus. Levofloxacin, tobramycin, clindamycin fusidic acid and cefazolin sodium were purchased from Dalian Meilun Biotechnology Co., Ltd.and Shenzhen China Resources Jiuxin Pharmaceutical Co., Ltd.. Ocular antibiotics susceptibility of multidrug-resistant Staphylococcus was determined by the Minimum Inhibitory Concentration methods according to the guidelines of Clinical and Laboratory Standards Institute.
Results :
35 methicillin-resistant Staphylococcus (MRS), 30 beta-lactamase-producing Staphylococcus (β-Lac) and 2 β-Lac MRS isolates were identified from 67 multidrug-resistant Staphylococcus. MRS isolates accounted for 80.00%,75.00%, 44.19% and 42.86%, and β-Lac isolates accounted for 20.00%, 20.00%, 53.49% and 42.86% in lacrimal sac, cornea, conjunctival sac and eyelid margin respectively. The drug-resistant Staphylococcus epidermidis was the major isolates in eyelid margin (57.14%) and conjunctival sac (46.51%). Meanwhile, the drug-resistant Staphylococcus aureus was the major isolates in lacrimal sac (40.00%) and cornea (33.33%). The susceptibility rates of cefazolin sodium, fusidic acid, levofloxacin, clindamycin and tobramycin against 35 MRS isolates were 74.29%, 68.57%, 34.29%, 34.29% and 31.43% respectively. The susceptibility rates of cefazolin sodium against MRS isolates were 100% in cornea, eyelid margin and lacrimal sac. The susceptibility rates of cefazolin sodium, fusidic acid, levofloxacin, tobramycin and clindamycin against 30 β-Lac isolates were 90.00%, 76.67%, 56.67%, 53.33% and 26.67% respectively. The susceptibility of cefazolin sodium and fusidic acid against β-Lac isolates were higher than MRS isolates.
Conclusions :
Our results indicate that cefazolin sodium and fusidic acid may be considered a reliable alternative for the treatment of multidrug-resistant staphylococcus in the ocular surface, especially of β-Lac drug-resistant staphylococcus.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.