June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Differential Expression of the SP Family Transcription Factors in Normal Human Lens and Cataract Patients
Author Affiliations & Notes
  • Jingmiao Wang
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Jialing Fu
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Yan Wang
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Shuyu Zheng
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • yuan xiao
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Jiawen Xiang
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Yuwen Gan
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Xingmiao Liang
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • David W Li
    Sun Yat-Sen University Zhongshan Ophthalmic Center, Guangzhou, Guangdong, China
  • Footnotes
    Commercial Relationships   Jingmiao Wang None; Jialing Fu None; Yan Wang None; Shuyu Zheng None; yuan xiao None; Jiawen Xiang None; Yuwen Gan None; Xingmiao Liang None; David Li None
  • Footnotes
    Support  NSFC#81970787, #82000876, #81770910, NSF-Guangdong Province and Guangzhou City Joint Program#20191515120014, ZOC-SKLO#3030901010110
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 4041 – F0005. doi:
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    • Get Citation

      Jingmiao Wang, Jialing Fu, Yan Wang, Shuyu Zheng, yuan xiao, Jiawen Xiang, Yuwen Gan, Xingmiao Liang, David W Li; Differential Expression of the SP Family Transcription Factors in Normal Human Lens and Cataract Patients. Invest. Ophthalmol. Vis. Sci. 2022;63(7):4041 – F0005.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The SP family transcription factors including SP1/SP3/SP4 play essential role in regulating lens differentiation through control of various crystallin genes [Gong et al., 2014. Proc. Natl. Acad. Sci. USA]. In our most recent studies, we also found that the SP family transcription factors also play an important role in the regulating transcription of the major heat shock protein HSP90β in lens epithelial cells. Whether these transcription factors are implicated in cataractogenesis remains to be further studied. In the present studies, we have analyzed the differential expression patterns of SP1/SP3/SP4 in normal human lens and cataract patients.

Methods : Normal human lens were collected from Guangdong Province Eye Bank. Cataract capsular epithelia were harvested during surgical operation with consent of cataract patients. Micro-Western blot analysis (Wes) was used to detect the expression levels of SP1/SP3/SP4.

Results : From normal human lens to cataractous lens, all SP1 family transcription factors were down-regulated. Among the different age groups of cataract patients, SP1 was upregulated over 50% from 50s to 60s. From 60s to 70s, however, SP1 was down-regulated more than 80%. From 70s to 80s, SP1 was upregulated to the level between 50s and 60s, but down-regulated over 80% again from 80s to 90s. In contrast, SP3 and SP4 remain relatively stable from 50s to 70s.

Conclusions : The SP family transcription factors display clearly differential expression patterns. Such patterns are consistent with the down-regulated expression of the major heat shock protein, HSP90β in lens epithelial cells. These results suggest that down-regulation of the SP family transcription factor level is implicated in cataractogenesis.Supported by National Natural Science Foundation of China (Grants #81970787, #82000876, #81770910) and Natural Science Foundation of Guangdong Province and Guangdong City Joint Program of China (Grant # 2019B1515120014), and the Fundamental Funds, 3030901010110 of the State Key Laboratory of Ophthalmology of Zhongshan Ophthalmic Center.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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